[Histonet] RE: PBA for Immunofluorescence

[Liz Chlipala]

We just use Dako's serum free protein block for IF.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: Nails, Felton [mailto:flnails <@t> texaschildrens.org] 
Sent: Thursday, May 20, 2010 1:29 PM
To: Liz Chlipala; Laurie Colbert; histonet <@t> lists.utsouthwestern.edu
Subject: PBA for Immunofluorescence

Concerning Immunofluorescence, where are you getting your (PBA) Protein
Blocking Agent from? I use to order it from Thermo Shandon but they no
longer carry it. 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Liz
Chlipala
Sent: Thursday, May 20, 2010 1:49 PM
To: Laurie Colbert; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Mounting Medium for Immunofluorescence

I think that the propidium iodide will stain the nuclei or DNA, similar
to DAPI, so this mounting media will counterstain the nuclei and its
visible in the FITC wavelength (488), while if you conterstain with DAPI
it is excited with a different wavelength, therefore it might interfere
with the FITC portion of your stain.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC
PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303)
682-9060 www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laurie
Colbert
Sent: Thursday, May 20, 2010 12:13 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Mounting Medium for Immunofluorescence

We normally coverslip our immunofluorescence slides with a permanent
aqueous mounting medium.  By mistake, today they were coverslipped with
a mounting medium that we use for coverslipping our FISH slides.  This
mounting medium is described as "Mounting medium for fluorescence, with
propidium iodide."

Will this make a difference when reading the slides??

 

Laurie Colbert

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------------------------------------------------
------
CONFIDENTIALITY NOTICE:
 The information in this e-mail may be confidential and/or
 privileged.  If you are not the intended recipient or an
 authorized representative of the intended recipient, you
 are hereby notified that any review, dissemination, or
 copying of this e-mail and its attachments, if any, or
 the information contained herein is prohibited.  If you
 have received this e-mail in error, please immediately
 notify the sender by return e-mail and delete this e-mail
 from your computer system.  Thank you.
========================================================================
======