[Histonet] PBA for Immunofluorescence

[Nails, Felton]

Concerning Immunofluorescence, where are you getting your (PBA) Protein Blocking Agent from? I use to order it from Thermo Shandon but they no longer carry it. 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Liz Chlipala
Sent: Thursday, May 20, 2010 1:49 PM
To: Laurie Colbert; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Mounting Medium for Immunofluorescence

I think that the propidium iodide will stain the nuclei or DNA, similar to DAPI, so this mounting media will counterstain the nuclei and its visible in the FITC wavelength (488), while if you conterstain with DAPI it is excited with a different wavelength, therefore it might interfere with the FITC portion of your stain.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
Sent: Thursday, May 20, 2010 12:13 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Mounting Medium for Immunofluorescence

We normally coverslip our immunofluorescence slides with a permanent aqueous mounting medium.  By mistake, today they were coverslipped with a mounting medium that we use for coverslipping our FISH slides.  This mounting medium is described as "Mounting medium for fluorescence, with propidium iodide."

Will this make a difference when reading the slides??

 

Laurie Colbert

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