[Histonet] Mounting Medium for Immunofluorescence

[Merced M Leiker]

Hi Laurie,

Propidium iodide is a DNA intercalator that fluoresces red (ex 488nm).

Regards,
Merced

--On Thursday, May 20, 2010 11:13 AM -0700 Laurie Colbert 
<laurie.colbert <@t> huntingtonhospital.com> wrote:

> We normally coverslip our immunofluorescence slides with a permanent
> aqueous mounting medium.  By mistake, today they were coverslipped with
> a mounting medium that we use for coverslipping our FISH slides.  This
> mounting medium is described as "Mounting medium for fluorescence, with
> propidium iodide."
>
> Will this make a difference when reading the slides??
>
>
>
> Laurie Colbert
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.