[Histonet] Mounting Medium for Immunofluorescence

[Merced M Leiker]

Actually if I could make a minor correction to your statement: propidium 
iodide is excited by green light at 488nm but emits in the red portion of 
the spectrum (620nm)...


--On Thursday, May 20, 2010 12:48 PM -0600 Liz Chlipala 
<liz <@t> premierlab.com> wrote:

> I think that the propidium iodide will stain the nuclei or DNA, similar
> to DAPI, so this mounting media will counterstain the nuclei and its
> visible in the FITC wavelength (488), while if you conterstain with DAPI
> it is excited with a different wavelength, therefore it might interfere
> with the FITC portion of your stain.
>
> Liz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Manager
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, Colorado 80308
> office (303) 682-3949
> fax (303) 682-9060
> www.premierlab.com
>
>
> Ship to Address:
> 1567 Skyway Drive, Unit E
> Longmont, Colorado 80504
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laurie
> Colbert
> Sent: Thursday, May 20, 2010 12:13 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Mounting Medium for Immunofluorescence
>
> We normally coverslip our immunofluorescence slides with a permanent
> aqueous mounting medium.  By mistake, today they were coverslipped with
> a mounting medium that we use for coverslipping our FISH slides.  This
> mounting medium is described as "Mounting medium for fluorescence, with
> propidium iodide."
>
> Will this make a difference when reading the slides??
>
>
>
> Laurie Colbert
>
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Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

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