[Histonet] Mounting Medium for Immunofluorescence

[Liz Chlipala]

I think that the propidium iodide will stain the nuclei or DNA, similar
to DAPI, so this mounting media will counterstain the nuclei and its
visible in the FITC wavelength (488), while if you conterstain with DAPI
it is excited with a different wavelength, therefore it might interfere
with the FITC portion of your stain.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laurie
Colbert
Sent: Thursday, May 20, 2010 12:13 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Mounting Medium for Immunofluorescence

We normally coverslip our immunofluorescence slides with a permanent
aqueous mounting medium.  By mistake, today they were coverslipped with
a mounting medium that we use for coverslipping our FISH slides.  This
mounting medium is described as "Mounting medium for fluorescence, with
propidium iodide."

Will this make a difference when reading the slides??

 

Laurie Colbert

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