[Histonet] weekend fixation

[LINDA MARGRAF]

 Histonetters:
Here's a message I was asked to post......

Dear Colleagues,
 I  have the following question concerning tissue processing. We do a lot of IHC work on NF fixed tissue. To standardize and minimize the effect of NF fixation, we fixate the tissue always for 24h. This is of course a problem for tissues taken on Friday. In the past, we asked our technicians to come on Saturday to embed the tissues in paraffin. Unfortunately, this is not possible anymore, and that is why I need your advice. What would you suggest ? 1) to leave the tissue in NF until Sunday evening and start processing, or 2) to keep the fixation time (24 hours) and leave the tissue in alcohol 70% until Sunday evening and then start processing.
 Thanks for your advice.
Kind regards,
Wim.
 

Prof. dr. Wim Van den Broeck, DVM, MSc, PhD
Cell biology and Histology
Department of Morphology - Faculty of Veterinary Medicine
Ghent University
Salisburylaan 133, B-9820 Merelbeke, BELGIUM



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