SPAM-LOW: Re: [Histonet] IHC

[Patsy Ruegg]

I do this all the time, but I do put a coverslip over the section and keep
it in a humid chamber.  We leave it on the counter with a diaper over it and
an ice pack sitting on top.

Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
andreahooper <@t> rocketmail.com
Sent: Friday, May 14, 2010 5:18 PM
To: sgoebel <@t> xbiotech.com; histonet-bounces <@t> lists.utsouthwestern.edu;
histonet <@t> lists.utsouthwestern.edu
Subject: SPAM-LOW: Re: [Histonet] IHC

Overnight is surely fine but make sure at 4 deg C for two reasons. First you
want to minimize any bacterial growth. Second you want to ensure it doesn't
evaporate significantly which would change your antibody concentration. For
that same reason make sure you humidify the chamber amply as well as don't
skimp on the volume applied to the slide - as even humidified and at 4 deg C
eventually everything evaporates (say if you left it for days).

If you routinely do this ab for 1h RT and are now switching to overnight be
aware you may (or may not) get more staining or nonspecific binding. The
titration of ab could be a little different when optimiZed for overnight.

That being said, I say go for it.

Sent from my Verizon Wireless BlackBerry

-----Original Message-----
From: sgoebel <@t> xbiotech.com
Date: Fri, 14 May 2010 14:37:19 
To: <histonet <@t> lists.utsouthwestern.edu>
Subject: [Histonet] IHC


   Can  you  leave  your  primary antibody on slides overnight (or the w
eekend)  or  will  this  screw  things  up?   I  don't want to be here
   anothercouple of hours for secondary, etc. on a Friday!!

   
   Sarah Goebel, B.A., HT (ASCP)

   <i>Histotechnician

   XBiotech USA Inc.

   8201 East Riverside Dr. Bldg 4 Suite 100

   Austin, Texas  78744

   (512)386-5107
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