[Histonet] RE: Histonet Digest, Vol 78, Issue 15

Wed May 12 10:17:34 CDT 2010

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-----Original Message-----
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Subject: Histonet Digest, Vol 78, Issue 15

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Today's Topics:

   1. Thanks for bone cutting suggestions - and anti-Rat CD3
      (Connolly, Brett M)
   2. Forwarding Request for Control Mehtod (WILLIAM DESALVO)
   3. RE: Thanks for bone cutting suggestions - and anti-Rat CD3
      (Liz Chlipala)
   4. CYP.07680 - cytology cross contamination (Brandi Higgins)
   5. L-DNAse II vs neutrophil (leukocyte) elastase inhibitor
      (Fabrice gankam)
   6. Genemed antibodies (Toine van der Aa)
   7. ICC (sgoebel <@t> xbiotech.com)
   8. RE: CYP.07680 - cytology cross contamination (Jeter, Brent)
   9. Labomed LX400 compound microscope (Steve Wong)
  10. RE: Forwarding Request for Control Mehtod (Tony Henwood)
  11. RE: Are Histotechs considered "exempt" employees? (Kim Tournear)
  12. RE: L-DNAse II vs neutrophil (leukocyte) elastase	inhibitor
      (Fabrice GANKAM)
  13. RE: Are Histotechs considered "exempt" employees? (Emmanuel Gonz)
  14. FW: [Histonet] Forwarding Request for Control Mehtod
      (Ian Montgomery)
  15. Re: FW: [Histonet] Forwarding Request for Control Mehtod
      (Lynette Pavelich)
  16. Servicing of Leica equipment in the UK (Krish Soni)
  17. Re: CYP.07680 - cytology cross contamination (DKBoyd <@t> chs.net)
  18. Auto Dialer for Thermo Excelsior (bsylinda <@t> aol.com)
  19. (no subject) (Kim Merriam)
  20. RE: Are Histotechs considered "exempt" employees?
      (sgoebel <@t> xbiotech.com)
  21. FFPE cell pellet prep (Kim Merriam)
  22. Xylene substitutes (Dawn Oakes)

----------------------------------------------------------------------

Message: 1
Date: Tue, 11 May 2010 13:37:58 -0400
From: "Connolly, Brett M" <brett_connolly <@t> merck.com>
Subject: [Histonet] Thanks for bone cutting suggestions - and anti-Rat
	CD3
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<63EA0607835FBA4689CEA9EA8B48269203126435 <@t> usctmx1141.merck.com>
Content-Type: text/plain; charset="us-ascii"

Thanks to all for your suggestions for cutting cross sections of cortical
bone. I have passed them along.

Now, what is the latest on CD3 IHC in FFPE rat tissues. I saw Ray Koelling's
suggestion in the archives using an Ab from BD Biosciences.
Is it cat # 550295?

What others if any do you suggest? HEIR ? protocol tips?

Thanks,
Brett

Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
tel. 215-652-2501 fax. 215-993-6803
brett_connolly <@t> merck.com

Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New
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affiliates is available at
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely for
the use of the individual or entity named on this message. If you are not
the intended recipient, and have received this message in error, please
notify us immediately by reply e-mail and then delete it from your system.

------------------------------

Message: 2
Date: Tue, 11 May 2010 12:00:56 -0600
From: WILLIAM DESALVO <wdesalvo.cac <@t> hotmail.com>
Subject: [Histonet] Forwarding Request for Control Mehtod
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BLU103-W22A6637493A094C96CBD5C91FA0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"

> I wonder if you could help me find someone who would kindly donate a 
> paraffin tissue block containing candida or other fungi to be used as 
> a control block. We have had difficulty in obtaining such tissue and 
> have tried all the hospitals in northern Ireland for a tissue block 
> suitable enough to act as a control. Many of the pathology departments 
> are in the same position as ourselves in that the control tissue being 
> used is getting in short supply.
> 
> I tried to make one using a culture from microbiology with fresh lambs 
> lung from a local abattoir but unfortunately the tissue seems to stain 
> with PAS as well and the candida where obscured although visible 
> probably due to post mortem changes in the tissue.

> Alternatively have any of your members successfully produced fungal 
> control material by other means.
> 
> Thanking you in advance

> Ian Clarke
> Biomedical Scientist
> Cellular Pathology Department
> Craigavon Area Hospital
> Sothern Health and Social Services Trust.
> 66 Lurgan Road
> Craigavon
> BT63 5QQ
> Northern Ireland

William DeSalvo, B.S., HTL(ASCP)

_________________________________________________________________
The New Busy is not the too busy. Combine all your e-mail accounts with
Hotmail.
http://www.windowslive.com/campaign/thenewbusy?tile=multiaccount&ocid=PID283
26::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_4

------------------------------

Message: 3
Date: Tue, 11 May 2010 12:32:00 -0600
From: "Liz Chlipala" <liz <@t> premierlab.com>
Subject: RE: [Histonet] Thanks for bone cutting suggestions - and
	anti-Rat CD3
To: "Connolly, Brett M" <brett_connolly <@t> merck.com>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<EE33BE5C905A3046A7FF8F58A64C8E4B1010C3 <@t> server.PremierLab.local>
Content-Type: text/plain;	charset="US-ASCII"

Dako has a rabbit polyclonal that works in rat.  The other thing with
the bone, is place the slide with a section on a flat hotplate (60 C)
until it turns clear - not too long or the articular cartilage will
flip.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com

Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Connolly, Brett M
Sent: Tuesday, May 11, 2010 11:38 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Thanks for bone cutting suggestions - and anti-Rat
CD3

Thanks to all for your suggestions for cutting cross sections of
cortical bone. I have passed them along.

Now, what is the latest on CD3 IHC in FFPE rat tissues. I saw Ray
Koelling's suggestion in the archives using an Ab from BD Biosciences.
Is it cat # 550295?

What others if any do you suggest? HEIR ? protocol tips?

Thanks,
Brett

Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
tel. 215-652-2501 fax. 215-993-6803
brett_connolly <@t> merck.com

Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you
are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
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------------------------------

Message: 4
Date: Tue, 11 May 2010 14:55:20 -0400
From: Brandi Higgins <brandihiggins <@t> gmail.com>
Subject: [Histonet] CYP.07680 - cytology cross contamination
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<AANLkTikwMwocnyrblyVcymF4M8NmefzYnvdYIdogX1hr <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Hello all,

For CAP policy CYP.07680 for "procedures to prevent cross-contamination of
specimens during processing and staining" - what are your labs doing?

 I work in a small lab so the histotechs process the cytology specimens and
the pathologists read the slides (we have no PA's or cytoprep techs or
cytotechs to screen slides).  We also process only non-GYN, so we don't have
to worry about GYN/non-GYN cross contamination.

The notes under this policy say procedures must prevent cross-contamination
between highly cellular specimens and suggest the screening method of
toluidine blue stain to determing if specimens are highly cellular.

Does anyone use the toluidine blue for this purpose?  If so could you tell
me the procedure for toluidine blue you use?  And how do you determing which
specimens you stain with toluidine blue and what qualifies as highly
cellular.  If so do you retain these toluidine blue slides for any period of
time?

CAP policy also suggests inserting a clean blank slide in each stain run and
examine for contamination.  Is anyone doing this?

We have been inspected before with no problems with this CAP question, but I
just want to make sure we are doing everything we can to prevent
cross-contamination.

Thanks in advance for your input!
Brandi Higgins, HT (ASCP)

------------------------------

Message: 5
Date: Tue, 11 May 2010 14:06:21 -0500
From: Fabrice gankam <gankam <@t> googlemail.com>
Subject: [Histonet] L-DNAse II vs neutrophil (leukocyte) elastase
	inhibitor
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<AANLkTil26ap8hPsOTqojfl--kfMfJqJ6NGZRn-kbAr4m <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Hi guys
Would like to know if any of you have used the L DNAse II or the serpin B1
antibody in the rat.
very few references mentioning this antibody. Sigma has one prestige
antibody for serpin B1 but do no know if it will work on rats.
I was wondering if the DNAse II is the equivalent of the L DNAse II (product
of the degradation of serpin)
thanks for your help

------------------------------

Message: 6
Date: Tue, 11 May 2010 21:14:24 +0200
From: Toine van der Aa <T.vanderAa <@t> sakura.eu>
Subject: [Histonet] Genemed antibodies
To: "'histonet <@t> lists.utsouthwestern.edu'"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:

<FEF9F39092D0674FBD928485A9ED979E01F6C86D1418 <@t> sakuramail2007.Sakura.local>

Content-Type: text/plain; charset=us-ascii

Hi,

Does anybody have experience with Genemed Biotechnologies antibodies and/or
probes of Histosonda?

Kind regards,

A.Van der Aa

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------------------------------

Message: 7
Date: Tue, 11 May 2010 12:16:33 -0700
From: sgoebel <@t> xbiotech.com
Subject: [Histonet] ICC
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:

<20100511121633.9e2d9aa830e8449a2412eb1e4f2f067e.6c1d241588.wbe <@t> email04.secu
reserver.net>

Content-Type: text/plain; charset="utf-8"

   Hello all,

   I am starting ICC and flourescence for  the first time.  I have done
   tons  of  IHC  in  the  past  with DAB and AEC   slightly confused with
the way the flourescence  works.  Do you have
   to  buy  a  secondary  that  is  already tagged, or is   system  where
it  attaches to the secondary like DAB does?    anyone have any advice or
some site where I can go to get some info on
   this.   Also,  what  do you use for positive and negative controls fo   r
ICC?

   Thanks fellow histo hotties!!

   [DEL: Sarah
   Histotechni   
   XBiotech USA Inc.

   8201 East Riverside Dr. Bldg 4 Suite 100

   Austin, Texas  78744
   (512)386-5107

------------------------------

Message: 8
Date: Tue, 11 May 2010 16:33:12 -0400
From: "Jeter, Brent" <brent.jeter <@t> gwu-hospital.com>
Subject: RE: [Histonet] CYP.07680 - cytology cross contamination
To: Brandi Higgins <brandihiggins <@t> gmail.com>,
	"histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:

<ABF1E22C45759E439FA2D6799086920D586FC878B8 <@t> CORP-EXVS03.corp.uhsinc.biz>

Content-Type: text/plain; charset="us-ascii"

Hi Brandi,

The clean, blank slide is some times inserted between cases in the staining
rack to determine if floaters are present.  It's sort of a retroactive test
- if you see cells on the blank slide, then you already have
cross-contamination and you need to re-prep and restain the cases
separately.  Some people feel that you've caught the floaters on your clean
slide and the problem is solved, but there's no guarantee others won't be
present on your specimen slides, too.

Toluidine blue is an example of a stain you could use to determine
cellularity; another option often used is a modified Wright Giemsa stain
such as Diff-Quik.  You're not looking to make a final diagnosis, but to
make a quick preparation and use a simple stain just to see if there are a
lot of cells present.  This is a more proactive test.  The idea is that
highly cellular specimens have an increased likelihood of shedding cells
into the stains and contaminating other cases; it also can be used to
identify obviously malignant cases, which should definitely be stained
separately.

If you're using a rapid stain to look for high cellularity, you need to
centrifuge and concentrate the specimen(s) first, just as you would in
regular prep.  The Diff-Quik stain is a Romanovsky stain and is used on
air-dried slides, so there's no need for fixation.

Hope that helps - 

Brent Jeter
Anatomic Pathology Supervisor
The George Washington University Hospital
202-715-5076 (phone)
202-715-4691 (fax)
brent.jeter <@t> gwu-hospital.com
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu
[histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Brandi Higgins
[brandihiggins <@t> gmail.com]
Sent: Tuesday, May 11, 2010 2:55 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] CYP.07680 - cytology cross contamination

Hello all,

For CAP policy CYP.07680 for "procedures to prevent cross-contamination of
specimens during processing and staining" - what are your labs doing?

 I work in a small lab so the histotechs process the cytology specimens and
the pathologists read the slides (we have no PA's or cytoprep techs or
cytotechs to screen slides).  We also process only non-GYN, so we don't have
to worry about GYN/non-GYN cross contamination.

The notes under this policy say procedures must prevent cross-contamination
between highly cellular specimens and suggest the screening method of
toluidine blue stain to determing if specimens are highly cellular.

Does anyone use the toluidine blue for this purpose?  If so could you tell
me the procedure for toluidine blue you use?  And how do you determing which
specimens you stain with toluidine blue and what qualifies as highly
cellular.  If so do you retain these toluidine blue slides for any period of
time?

CAP policy also suggests inserting a clean blank slide in each stain run and
examine for contamination.  Is anyone doing this?

We have been inspected before with no problems with this CAP question, but I
just want to make sure we are doing everything we can to prevent
cross-contamination.

Thanks in advance for your input!
Brandi Higgins, HT (ASCP)
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

UHS Confidentiality Notice:  This e-mail message, including any attachments,
is for the sole use of the intended recipient (s) and may contain
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sent to you in error, please notify the sender by reply e-mail and destroy
all copies of the original message.

------------------------------

Message: 9
Date: Tue, 11 May 2010 15:53:31 -0500
From: "Steve Wong" <steve <@t> myelinrepair.org>
Subject: [Histonet] Labomed LX400 compound microscope
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<F18F544AC66AF8439B43B789622BBF24EE5BE1 <@t> EXVS03.wng.chicago.hostway>
Content-Type: text/plain;	charset="us-ascii"

Hello,

Does anyone have any experience with that Labomed Lx 400 compound
microscope?  It is new and it is cheap (scope with Moticam 2000 camera -
~$2,000), but I cannot find any reviews on it.

I'm looking for a simple microscope to take digital pictures of cross
sections of rat spinal cords for some volumetric measurements.

Steve

------------------------------

Message: 10
Date: Wed, 12 May 2010 10:53:48 +1000
From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
Subject: RE: [Histonet] Forwarding Request for Control Mehtod
To: "WILLIAM DESALVO" <wdesalvo.cac <@t> hotmail.com>, "histonet"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <AE22DCD58FA1B045B26FF61D7259578F1CB3FD <@t> x2k3.nch.kids>
Content-Type: text/plain; charset="us-ascii"

Ian,

What I have done in the past is to raid the Staff Fridge (or your son's
bedroom!).
There is usually old food left there that has gone moldy - bread or even
strawberries.
Wearing gloves & in a fume hood slice the bread or fruit into slices
(ensuring visible growth of fungi is present), fix for two or more days
in NBF, process as usual.

Voila - fungi controls

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of WILLIAM
DESALVO
Sent: Wednesday, 12 May 2010 4:01 AM
To: histonet
Subject: [Histonet] Forwarding Request for Control Mehtod

> I wonder if you could help me find someone who would kindly donate a 
> paraffin tissue block containing candida or other fungi to be used as 
> a control block. We have had difficulty in obtaining such tissue and 
> have tried all the hospitals in northern Ireland for a tissue block 
> suitable enough to act as a control. Many of the pathology departments

> are in the same position as ourselves in that the control tissue being

> used is getting in short supply.
> 
> I tried to make one using a culture from microbiology with fresh lambs

> lung from a local abattoir but unfortunately the tissue seems to stain

> with PAS as well and the candida where obscured although visible 
> probably due to post mortem changes in the tissue.

> Alternatively have any of your members successfully produced fungal 
> control material by other means.
> 
> Thanking you in advance

> Ian Clarke
> Biomedical Scientist
> Cellular Pathology Department
> Craigavon Area Hospital
> Sothern Health and Social Services Trust.
> 66 Lurgan Road
> Craigavon
> BT63 5QQ
> Northern Ireland

William DeSalvo, B.S., HTL(ASCP)

_________________________________________________________________
The New Busy is not the too busy. Combine all your e-mail accounts with
Hotmail.
http://www.windowslive.com/campaign/thenewbusy?tile=multiaccount&ocid=PI
D28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_4___________________________
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------------------------------

Message: 11
Date: Tue, 11 May 2010 19:24:49 -0700 (PDT)
From: Kim Tournear <kimtournear <@t> yahoo.com>
Subject: RE: [Histonet] Are Histotechs considered "exempt" employees?
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <235254.69116.qm <@t> web54207.mail.re2.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Well said Andi!!

~Kim Tournear ~ HT (ASCP), QIHC (ASCP)
Histology Supervisor
Tucson Medical Center
Tucson,  AZ
~Don't let your life end before it begins~
OU Rocks!!!!

--- On Tue, 5/11/10, Heckford, Karen - SMMC-SF <Karen.Heckford <@t> CHW.edu>
wrote:

From: Heckford, Karen - SMMC-SF <Karen.Heckford <@t> CHW.edu>
Subject: RE: [Histonet] Are Histotechs considered "exempt" employees?
To: "Andrea Grantham" <algranth <@t> email.arizona.edu>
Cc: histonet <@t> lists.utsouthwestern.edu
Date: Tuesday, May 11, 2010, 8:17 AM

I am sooooo with you on this one.   HT's need to start demanding and
higher wages everywhere.  We are a rare breed with special talents.
Without a good tech you do not get good slides!!  We are not a dime a
dozen.  AUGH!  

I know just a hour north of where I am the wages can be $10-$15.00 less.
Not much difference in living expenses either.

Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Andrea
Grantham
Sent: Tuesday, May 11, 2010 7:35 AM
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Are Histotechs considered "exempt" employees?

Anthony,
You live in CA, work 50 hour weeks and you are a HTL with an  
unprecedented and valuable resource at your disposal, Histonet and you  
know how to use it. For this you get paid $16.15/hour by what appears  
to be an employer who isn't grateful to have you. I'd start looking  
for another job.
Just my humble opinion.

Andi

Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cell Biology and Anatomy
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algranth <@t> email.arizona.edu
Tel: 520.626.4415     Fax: 520.626.2097

"happy slicing and dicing and may all your stains work perfectly" -  
Paula Sicurello
P Please consider the environment before printing this email.

On May 10, 2010, at 9:00 PM, Anthony Sandoval wrote:

> Hello fellow Histotechs, I have recently become certified as an HTL  
> and was wondering if anyone out there is an 'exempt' employee? I  
> live in California and feel that I am being taken advantage of.  I  
> make 16.15$ per hour and frequently work 50 hour weeks. Am I off  
> base? should I just be grateful that I have a job, as my employer so  
> frequently reminds me? Thank you Histonet! you have been an  
> invaluable resource in my career and assisting me in passing the HTL!
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 12
Date: Wed, 12 May 2010 04:25:45 +0200
From: Fabrice GANKAM <gankam <@t> googlemail.com>
Subject: [Histonet] RE: L-DNAse II vs neutrophil (leukocyte) elastase
	inhibitor
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <3CC647ECDCDC41E1BC06895D398ABF38 <@t> PCdeGANKAM>
Content-Type: text/plain;	charset="US-ASCII"

Hi guys

Would like to know if any of you have used the L DNAse II or the serpin B1
antibody in the rat.

very few references mentioning this antibody. Sigma has one prestige
antibody for serpin B1 but do no know if it will work on rats.

I was wondering if the DNAse II is the equivalent of the L DNAse II (product
of the degradation of serpin)

thanks for your help

------------------------------

Message: 13
Date: Wed, 12 May 2010 00:20:21 -0700
From: Emmanuel Gonz <emmanuelg <@t> gmail.com>
Subject: [Histonet] RE: Are Histotechs considered "exempt" employees?
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<AANLkTin70gQBDmNm2FIYOBnwk2_on_lhyEuLwPiFZu6X <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

>From what i know and heard, your facility is definitely getting a great
deal. It really depends on the facility. The lab i work at starts
non-certified techs around 20. I know of a small derm lab that start
non-certified techs around 18.

Emmanuel Gonzaga HT(ASCP)

------------------------------

Message: 14
Date: Wed, 12 May 2010 12:31:37 +0100
From: "Ian Montgomery" <ian.montgomery <@t> bio.gla.ac.uk>
Subject: FW: [Histonet] Forwarding Request for Control Mehtod
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <FC7C4BA7E22D4F158D2547E26F6CE25A <@t> IBLS.GLA.AC.UK>
Content-Type: text/plain;	charset="us-ascii"

Tony,
	Is this a universal teenage thing, filthy bedrooms? I'm convinced
there are alien life forms in my daughter's bedroom. They make the strangest
noises, grunts and groan and "I'm bored." Me, I was the perfect teenager
apart from the Celtic thing of taking to the drink and of course, lusting
after girls.
Ian. 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tony Henwood
Sent: 12 May 2010 01:54
To: WILLIAM DESALVO; histonet
Subject: RE: [Histonet] Forwarding Request for Control Mehtod

Ian,

What I have done in the past is to raid the Staff Fridge (or your son's
bedroom!).
There is usually old food left there that has gone moldy - bread or even
strawberries.
Wearing gloves & in a fume hood slice the bread or fruit into slices
(ensuring visible growth of fungi is present), fix for two or more days
in NBF, process as usual.

Voila - fungi controls

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of WILLIAM
DESALVO
Sent: Wednesday, 12 May 2010 4:01 AM
To: histonet
Subject: [Histonet] Forwarding Request for Control Mehtod

> I wonder if you could help me find someone who would kindly donate a 
> paraffin tissue block containing candida or other fungi to be used as 
> a control block. We have had difficulty in obtaining such tissue and 
> have tried all the hospitals in northern Ireland for a tissue block 
> suitable enough to act as a control. Many of the pathology departments

> are in the same position as ourselves in that the control tissue being

> used is getting in short supply.
> 
> I tried to make one using a culture from microbiology with fresh lambs

> lung from a local abattoir but unfortunately the tissue seems to stain

> with PAS as well and the candida where obscured although visible 
> probably due to post mortem changes in the tissue.

> Alternatively have any of your members successfully produced fungal 
> control material by other means.
> 
> Thanking you in advance

> Ian Clarke
> Biomedical Scientist
> Cellular Pathology Department
> Craigavon Area Hospital
> Sothern Health and Social Services Trust.
> 66 Lurgan Road
> Craigavon
> BT63 5QQ
> Northern Ireland

William DeSalvo, B.S., HTL(ASCP)

_________________________________________________________________
The New Busy is not the too busy. Combine all your e-mail accounts with
Hotmail.
http://www.windowslive.com/campaign/thenewbusy?tile=multiaccount&ocid=PI
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------------------------------

Message: 15
Date: Wed, 12 May 2010 08:28:00 -0400
From: "Lynette Pavelich" <lpaveli1 <@t> hurleymc.com>
Subject: Re: FW: [Histonet] Forwarding Request for Control Mehtod
To: "Ian Montgomery" <ian.montgomery <@t> bio.gla.ac.uk>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4BEA6690.59CD.00EE.0 <@t> hurleymc.com>
Content-Type: text/plain; charset=US-ASCII

If you have one of those great automatic stainers that has a drain tube
going into a sink, you will also find that, after some time, a wonderful
fungus will grow inside of the tubing!! We remove the tubing and shake
the fungus loose.  Counter staining isn't the best on this type of
specimen, but when you're in a pinch, you will definitely obtain
positive fungus staining!

And, wherever you are, it must be warmer/dryer than Michigan right
now..........please send heat!!!

hope this helped!
Lynette

Lynette Pavelich, HT(ASCP)
Histology Supervisor
Hurley Medical Center
One Hurley Plaza
Flint, MI  48503
email: Lpaveli1 <@t> hurleymc.com
ph:  810-257-9948
Lab:  810-257-9138
fax:  810-762-7082

>>> "Ian Montgomery" <ian.montgomery <@t> bio.gla.ac.uk> 5/12/2010 7:31 AM
>>>
Tony,
	Is this a universal teenage thing, filthy bedrooms? I'm
convinced
there are alien life forms in my daughter's bedroom. They make the
strangest
noises, grunts and groan and "I'm bored." Me, I was the perfect
teenager
apart from the Celtic thing of taking to the drink and of course,
lusting
after girls.
Ian. 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tony
Henwood
Sent: 12 May 2010 01:54
To: WILLIAM DESALVO; histonet
Subject: RE: [Histonet] Forwarding Request for Control Mehtod

Ian,

What I have done in the past is to raid the Staff Fridge (or your
son's
bedroom!).
There is usually old food left there that has gone moldy - bread or
even
strawberries.
Wearing gloves & in a fume hood slice the bread or fruit into slices
(ensuring visible growth of fungi is present), fix for two or more
days
in NBF, process as usual.

Voila - fungi controls

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
WILLIAM
DESALVO
Sent: Wednesday, 12 May 2010 4:01 AM
To: histonet
Subject: [Histonet] Forwarding Request for Control Mehtod

> I wonder if you could help me find someone who would kindly donate a

> paraffin tissue block containing candida or other fungi to be used as

> a control block. We have had difficulty in obtaining such tissue and

> have tried all the hospitals in northern Ireland for a tissue block 
> suitable enough to act as a control. Many of the pathology
departments

> are in the same position as ourselves in that the control tissue
being

> used is getting in short supply.
> 
> I tried to make one using a culture from microbiology with fresh
lambs

> lung from a local abattoir but unfortunately the tissue seems to
stain

> with PAS as well and the candida where obscured although visible 
> probably due to post mortem changes in the tissue.

> Alternatively have any of your members successfully produced fungal 
> control material by other means.
> 
> Thanking you in advance

> Ian Clarke
> Biomedical Scientist
> Cellular Pathology Department
> Craigavon Area Hospital
> Sothern Health and Social Services Trust.
> 66 Lurgan Road
> Craigavon
> BT63 5QQ
> Northern Ireland

William DeSalvo, B.S., HTL(ASCP)

_________________________________________________________________
The New Busy is not the too busy. Combine all your e-mail accounts
with
Hotmail.
http://www.windowslive.com/campaign/thenewbusy?tile=multiaccount&ocid=PI

D28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_4___________________________
____________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

****************************************************************************
*****
This email and any files transmitted with it are confidential and
intended
solely for the use of the individual or entity to whom they are
addressed.
If you are not the intended recipient, please delete it and notify the
sender.

Views expressed in this message and any attachments are those of the
individual sender, and are not necessarily the views of The Children's
Hospital at Westmead

This note also confirms that this email message has been virus scanned
and
although no computer viruses were detected, The Childrens Hospital at
Westmead accepts no liability for any consequential damage resulting
from
email containing computer viruses.
****************************************************************************
*****

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 16
Date: Wed, 12 May 2010 13:34:11 +0100
From: Krish Soni <m0702936 <@t> sgul.ac.uk>
Subject: [Histonet] Servicing of Leica equipment in the UK
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <E55B8C80-F018-4CB3-A30F-EF9B085E62AF <@t> sgul.ac.uk>
Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes

We are a histology lab based in the UK.  We have a Leica CV5000  
coverslipper and a Leica Autostainer XL.

We are trying to find an independent company or operator (other than  
Leica) than can service our Leica equipment for us.

Does anyone know a company or ex Leica engineer we could call?

Thanks,

Chris

m0702936 <@t> sgul.ac.uk

------------------------------

Message: 17
Date: Wed, 12 May 2010 08:42:08 -0400
From: DKBoyd <@t> chs.net
Subject: Re: [Histonet] CYP.07680 - cytology cross contamination
To: Brandi Higgins <brandihiggins <@t> gmail.com>
Cc: histonet <@t> lists.utsouthwestern.edu,
	histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
	<OF30D796F7.17E80F54-ON85257721.00440C64-85257721.0045C74E <@t> chs.net>
Content-Type: text/plain;	charset="US-ASCII"

Brandi,
Body effusions have a high potential for cross contamination during the 
staining procedure.  Body fluids ie:  pleural fluid, peritoneal fluid, 
etc.  For that reason they are not stained with other non-gyn specimens 
ie:  bronch. wash, esoph. brush, CSF etc.  Also after the staining all 
solutions up to the 95% before the OG-6 are dumped and refilled.
The Hematoxylin, OG-6 and EA65 are filtered.  The 95% after the EA-65 is 
dumped and alcohols are rotated up.
This should prevent cross contamination.  Written documentation should be 
your procedure and proof that you are changing/filtering solutions.

Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical 
Center I 
200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l F: 
804-765-5582 l dkboyd <@t> chs.net

Brandi Higgins <brandihiggins <@t> gmail.com> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
05/11/2010 03:28 PM

To
histonet <@t> lists.utsouthwestern.edu
cc

Subject
[Histonet] CYP.07680 - cytology cross contamination

Hello all,

For CAP policy CYP.07680 for "procedures to prevent cross-contamination of
specimens during processing and staining" - what are your labs doing?

 I work in a small lab so the histotechs process the cytology specimens 
and
the pathologists read the slides (we have no PA's or cytoprep techs or
cytotechs to screen slides).  We also process only non-GYN, so we don't 
have
to worry about GYN/non-GYN cross contamination.

The notes under this policy say procedures must prevent 
cross-contamination
between highly cellular specimens and suggest the screening method of
toluidine blue stain to determing if specimens are highly cellular.

Does anyone use the toluidine blue for this purpose?  If so could you tell
me the procedure for toluidine blue you use?  And how do you determing 
which
specimens you stain with toluidine blue and what qualifies as highly
cellular.  If so do you retain these toluidine blue slides for any period 
of
time?

CAP policy also suggests inserting a clean blank slide in each stain run 
and
examine for contamination.  Is anyone doing this?

We have been inspected before with no problems with this CAP question, but 
I
just want to make sure we are doing everything we can to prevent
cross-contamination.

Thanks in advance for your input!
Brandi Higgins, HT (ASCP)
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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Disclaimer: This electronic message may contain information that is
Proprietary, Confidential, or legally privileged or protected. It
is intended only for the use of the individual(s) and entity named
in the message. If you are not an intended recipient of this
message, please notify the sender immediately and delete the
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------------------------------

Message: 18
Date: Wed, 12 May 2010 09:15:18 -0400
From: bsylinda <@t> aol.com
Subject: [Histonet] Auto Dialer for Thermo Excelsior
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <8CCBFEC4D4344E6-C18-7D3D <@t> webmail-d001.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"

Hello Histo,

Is there anyone out there with any experience of installing a remote voice
dialer on the processor.  We have have one and think that it has possibly
been fried due to a power surge.  However this one was purchased years ago
at Radio Shack and they no longer sell the same dialer.  The dialers that I
currently see are wireless, can anyone out there with experience with these
wireless devices give me any suggestions.

Thanks in advance,
Sylinda Battle, HT (ASCP)

------------------------------

Message: 19
Date: Wed, 12 May 2010 06:24:06 -0700 (PDT)
From: Kim Merriam <kmerriam2003 <@t> yahoo.com>
Subject: [Histonet] (no subject)
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <700229.76256.qm <@t> web50303.mail.re2.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Good morning,

I am trying to make some nice FFPE cell pellet blocks, but I seem to lose a
lot of cells along the way (especially when trying to take the cells out of
the tube).  We are fixing the cells in NBF, spinning them down, adding 70%
and spinning down again.  At that point, I am trying to scoop out the cells
(with a weighing scoop) and wrap them in lens paper for processing.  I am
losing a lot of the cells during this step, because the pellet is not quite
solid.  Do you think it would be OK to let the cells air-dry a bit and then
take them out for processing?  I know this goes against everything I was
taught in histology, but I am really at a loss here.

Anyone have any hot tips for me?

Kim
 Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA 

------------------------------

Message: 20
Date: Wed, 12 May 2010 06:46:28 -0700
From: sgoebel <@t> xbiotech.com
Subject: RE: [Histonet] Are Histotechs considered "exempt" employees?
To: "Kim Tournear" <kimtournear <@t> yahoo.com>
Cc: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:

<20100512064628.9e2d9aa830e8449a2412eb1e4f2f067e.af45b5d3d8.wbe <@t> email04.secu
reserver.net>

Content-Type: text/plain; charset="utf-8"

   So, I had the same problem a few years ago.  They are totall   advantage
of  you.  I live in Austin where I can't imagine tha   cost  of living is
more than CA.  The average here is between 20    25 an hour, sometimes
higher if you can get a MOHS job at a priva   derm  clininc.   Start
looking, usually in my experience histo. jobs    are  everywhere,  but
sometimes  you just have to call around.  Also,
   usu   wise)  about  HT   histology department.  
   Happy hunting!!

   Sarah Goebel, B.A., HT (ASCP)

   <
   Histo   
   XBiotech USA Inc.

   8201 East Rivers
   Austin, Texas  78744
   (512)386-5107

   -------- Original Message --------
   Subject: RE: [Histonet] Are Histotechs considered "exempt" employees?
   From: Kim Tournear <kimtournear <@t> yahoo.com>
   Date: Tue, May 11, 2010 7:24 pm
   To: Histonet <histonet <@t> lists.utsouthwestern.edu>
   Well said Andi!!
   ~Kim Tournear ~ HT (ASCP), QIHC (ASCP)
   Histology Supervisor
   Tucson Medical Center
   Tucson,  AZ
   ~Don't let your life end before it begins~
   OU Rocks!!!!
   ---     On     Tue,     5/11/10,    Heckford,    Karen    -    SMMC-SF
   <Karen.Heckford <@t> CHW.edu&g   From: Heckford, Karen - SMMC-SF
<Karen.Heckford <@t> CHW.edu>
   Subject: RE: [Histonet] Are Histotechs considered "exempt" employees?
   To: "Andrea Grantham" <algranth <@t> email.arizona.edu>
   Cc: histonet <@t> lists.utsouthwestern.edu
   Date: Tuesday, May 11, 2010, 8:17 AM
   I am sooooo with you on this one.   HT's need to start deman   higher
wages everywhere.  We are a rare breed with special talents.
   Without  a  good  tech you do not get good slides!!  We are not a dime
   a<   I  know  just  a  hour north of where I am the wages can be
$10-$15.00
   less.
   Not much difference in living expenses either.
   Karen Heckford HT ASCP CE
   Lead Histology Technician
   St. Mary's Medical Center
   450 Stanyan St.
   San Francisco, Ca. 94117
   415-668-1000 ext. 6167
   -----Original Message-----
   From: histonet-bounces <@t> lists.utsouthwestern.edu
   [[1]mailto:histonet-bounces <@t> lists.utsouthwestern.e   Andrea
   Grantham
   Sent: Tuesday, May 11, 2010 7:35 AM
   Cc: histonet <@t> lists.utsouthwestern.edu
   Subject: Re: [Histonet] Are Histotechs considered "exempt" employees?
   Anthony,
   You live in CA, work 50 hour weeks and you are a HTL with an
   unprecedented  and  valuable  resource  at your disposal, Histonet and
   you    know  how  to  use  it.  For  this  you  get  paid $16.15/hour by
what
   appears    to  be  an  employer who isn't grateful to have you. I'd start
looking
   <   Just my humble opinion.
   Andi
   Andrea Grantham, HT (ASCP)
   Senior Research Specialist
   University of Arizona
   Cell Biology and Anatomy
   Histology Service Laboratory
   P.O.Box 245044
   Tucson, AZ 85724
   algranth <@t> email.arizona.edu
   Tel: 520.626.4415     Fax: 520.626.2097
   "happy  slicing  and  dicing and may all your stains work perfectly" -
   <   P Please consider the environment before printing this email.
   On May 10, 2010, at 9:00 PM, Anthony Sandoval wrote:
   >  Hello  fellow  Histotechs,  I  have recently become certified as an
   HTL&nb   > and was wondering if anyone out there is an 'exempt' employee?
I    >  live  in California and feel that I am being taken advantage of.
I
   > make 16.15$ per hour and frequently work 50 hour weeks. Am I off    >
base?  should  I just be grateful that I have a job, as my employer
   so&n   > frequently reminds me? Thank you Histonet! you have been an
   >  invaluable  resource  in  my career and assisting me in passing the
   HTL!<   > _______________________________________________
   > Histonet mailing list
   > Histonet <@t> lists.utsouthwestern.edu
   > [2]http://lists.utsouthwestern.edu/mailman/listinfo/histonet   >
   _______________________________________________
   Histonet mailing list
   Histonet <@t> lists.utsouthwestern.edu
   [3]http://lists.utsouthwestern.edu/mailman/listinfo/histonet<
_______________________________________________
   Histonet mailing list
   Histonet <@t> lists.utsouthwestern.edu
   [4]http://lists.utsouthwestern.edu/mailman/listinfo/histonet<
_______________________________________________
   Histonet mailing list
   Histonet <@t> lists.utsouthwestern.edu
   [5]http://lists.utsouthwestern.edu/mailman/listinfo/histonet<
References

   1. file://localhost/tmp/3D"#Compose"
   2. 3D"http://lists.utsouthwestern.edu/mailman/l   3.
3D"http://lists.utsouthwestern.edu/mailman/listin   4.
3D"http://lists.utsouthwestern.edu/mailman/listin   5.
3D"http://lists.utsouthwestern.edu/mailman/listin

------------------------------

Message: 21
Date: Wed, 12 May 2010 06:50:14 -0700 (PDT)
From: Kim Merriam <kmerriam2003 <@t> yahoo.com>
Subject: [Histonet] FFPE cell pellet prep
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <491802.22615.qm <@t> web50306.mail.re2.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Sorry - I forgot to put a subject line.

Good morning,

I am trying to make some nice FFPE cell pellet blocks, but I seem to lose a
lot of cells along the way (especially when trying to take the cells out of
the tube).  We are fixing the cells in NBF, spinning them down, adding 70%
and spinning down again.  At that point, I am trying to scoop out the cells
(with a weighing scoop) and wrap them in lens paper for processing.  I am
losing a lot of the cells during this step, because the pellet is not quite
solid.  Do you think it would be OK to let the cells air-dry a bit and then
take them out for processing?  I know this goes against everything I was
taught in histology, but I am really at a loss here.

Anyone have any hot tips for me?

Kim
 Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA 

------------------------------

Message: 22
Date: Wed, 12 May 2010 07:02:31 -0700
From: Dawn Oakes <doakes <@t> olympicmedical.org>
Subject: [Histonet] Xylene substitutes
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:

<F5ECF3459F04CB43850D1EAC8180A737E33538 <@t> is-210vs.olympicmedical.local>
Content-Type: text/plain; charset="us-ascii"

What are labs using for xylene substitute. I will be using it in a hand
staining setup for Mohs. Feed back on  Histo-Clear,  Clear solve, S-3
and formular 83.

Thanks in advance.

Dawn Oakes HT

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