[Histonet] (no subject)

[sgoebel <@t> xbiotech.com]

   I  know the histogel is pretty expensive (1500ish per kit), try ju   plain  agar  powder and mix with water...it is super cheap that way.    Happy spinning!!  =)

   Sarah Goebel, B.A., HT (ASCP)
   H   XBiotech USA Inc.
   8201 East Ri      -------- Original Message --------
   Subject: Re: [Histonet] (no subject)
   From: Kim Merriam <kmerriam2003 <@t> yahoo.com>
   Date: Wed, May 12, 2010 7:49 am
   To: "Cormier, Kathleen" <Kathleen.Cormier <@t> crl.com>
   Cc: Histonet <histonet <@t> lists.utsouthwestern.edu>, sgoebel <@t> xbiotech.co   m
   Thanks  for  the  tips  everyone!   I  am  going  to order some aga   (histogel)  and  use  it!  We are all very excited around here abo   this, we have been pulling our hair out over these darn pellets.

   Kim Merriam, MA, HT(ASCP)QIHC
   Cambridge, MA
      <     _________________________________________________________________

   From: "Cormier, Kathleen" <Kathleen.Cormier <@t> cr   To: Kim Merri      Subject: RE: [Histonet] (no subject)
      Have  you used Histogel? I have used it in the past (other pl   employment)  and  on samples like yours. I just bought the gel, (not    the  whole  unit,  just  the gel) scooped some out of the tube, placed
   into  a w   took a transfer   the  tube/pellet  on  a   then  popped  out  the  now  solid   baggie/paper) in a cassette. Works like   Kathy
   Kathy Cormier
   Histology Manager
      Charles River Laboratories
   251 Ballardvale Street
   Wilmin   Ph: 781-222-6803
   Fax: 978-988-8793
   [1]kathleen.cormier <@t> crl.com
   The     Science  will be   get more informa   Accelerating Drug Development. Exactly.
   N   confidential   and   m   information.  You  must  not  disclose   without  Charles River's express written cons   intended recipient you must not copy, distribute or   the information contained in it for any purpose other th   us.  If  you  have  received  this  message  in error, please notify    Charles River immediately, and delete it from your system.
   -----Orig   From:                     [2]histonet-bounces <@t> lists.utsouthwestern.edu
   [mailto:[3]histonet-bounc   Kim Merriam
   Sent: Wednesda   To: [4]histonet <@t> lists.utsouthwestern.edu
   Subject: [Histonet] (no subje   Good morning,
   I am trying to make some nice FFPE cel   lose  a  lot  of cells along the way (espe   the  cells  out  of  the  tube).   We  are  fixing    spinning  them  down,  adding  70% and spinning down again.&n   that  point,  I am trying to scoop out the cells (with a weighing sco   op)  and wrap them in lens paper for processing.  I am losing a lot of
      Do   take  them   was taught   Anyone have any hot t   Kim
    Kim Merriam, MA, HT(ASCP)QIHC
   Cambridge,
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References

   1. 3D"mailto:kathleen.cormier <@t> crl.com"
   2. 3D"mailto:histonet-bou   3. 3D"mailto:histonet-bounces <@t> lists.utsouthwestern.edu"
   4. 3D"mailto:histonet   5. file://localhost/tmp/3D"mail   6. 3D"http://=/
   7. 3D"http://www.criver.com"/
   8. 3D"http://www.criver.com/"