[Histonet] (no subject)

Wed May 12 09:49:49 CDT 2010

Thanks for the tips everyone!  I am going to order some agar (histogel) and use it!  We are all very excited around here about this, we have been pulling our hair out over these darn pellets.
 Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA 

________________________________
From: "Cormier, Kathleen" <Kathleen.Cormier <@t> crl.com>
To: Kim Merriam <kmerriam2003 <@t> yahoo.com>
Sent: Wed, May 12, 2010 10:40:19 AM
Subject: RE: [Histonet] (no subject)

Hi Kim,

Have you used Histogel? I have used it in the past (other places of employment) and on samples like yours. I just bought the gel, (not the whole unit, just the gel) scooped some out of the tube, placed into a weight boat, microwaves for a few seconds until liquid, then took a transfer pipet and dropped onto the cell pellet. I then placed the tube/pellet on a cold plate or fridge and wait until solid. I then popped out the now solid pellet, and processed as usual (no baggie/paper) in a cassette. Works like a charm....

Kathy

Kathy Cormier

Histology Manager

Charles River Laboratories

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Ph: 781-222-6803

Fax: 978-988-8793

kathleen.cormier <@t> crl.com

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kim Merriam
Sent: Wednesday, May 12, 2010 9:24 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Good morning,

I am trying to make some nice FFPE cell pellet blocks, but I seem to lose a lot of cells along the way (especially when trying to take the cells out of the tube).  We are fixing the cells in NBF, spinning them down, adding 70% and spinning down again.  At that point, I am trying to scoop out the cells (with a weighing scoop) and wrap them in lens paper for processing.  I am losing a lot of the cells during this step, because the pellet is not quite solid.  Do you think it would be OK to let the cells air-dry a bit and then take them out for processing?  I know this goes against everything I was taught in histology, but I am really at a loss here.

Anyone have any hot tips for me?

Kim
 Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA 

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