[Histonet] Responses to IHC CAP Validation question

[BSullivan <@t> shorememorial.org]

One important thing to remember is that you should make sure All material
being used for validation is processed the same way. Will your control
tissue be purchased or processed in your lab? Control tissue is used for
validation but you should use tissue with various levels of positivity.

Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
AP Supervisor
Shore Memorial Hospital
609-653-3590


                                                                           
             tonia.richmond <@t> gr                                             
             acepathology.com                                              
             Sent by:                                                   To 
             histonet-bounces@         "McMahon, Loralee A"                
             lists.utsouthwest         <Loralee_Mcmahon <@t> URMC.Rochester.edu 
             ern.edu                   >                                   
                                                                        cc 
                                       "histonet <@t> lists.utsouthwestern.edu" 
             05/06/2010 11:27          <histonet <@t> lists.utsouthwestern.edu> 
             AM                        , "thisisann <@t> aol.com"               
                                       <thisisann <@t> aol.com>                 
                                                                   Subject 
                                       RE: [Histonet] Responses to IHC CAP 
                                       Validation question                 
                                                                           
                                                                           
                                                                           
                                                                           
                                                                           
                                                                           





   If  you  are a brand new lab, how do you validate IHC if you are no= t
   yet  receiving patient specimens?  Can the validation be done on cont
rol tissues only?




   Sincerely,


   Tonia Richmond, AS, HT (ASCP)
   Chief Operations Officer = / Laboratory
   Grace Pathology
   PH:  (501) 765-7367
   Email: = [1]tonia.= richmond <@t> gracepathology.com



   -----histonet-bounces <@t> lists.utsouthwestern.edu wrote: -----
   <= /FONT>

     To:        "thisisann@=        aol.com"        <thisisann <@t> aol.com>,
     "histonet <@t> lists.utsouthwestern.edu" <
;histonet <@t> lists.utsouthwestern.edu>
     From: "McMahon, Loralee A" <Lo= ralee_Mcmahon <@t> URMC.Rochester.edu>
     Sent by: histonet-bounces <@t> lists.u= tsouthwestern.edu
     Date: 04/28/2010 02:01PM
     Subject: RE: [Histonet] Re= sponses to IHC CAP Validation question
     Any  inspection  that  I have under= gone we have used the 25 to 30
     case  rule.   Except  for  the Er/Pr//Her-2= .  We use closer to 50
     cases.   We  also  use  a  TMA to make our live= s easier.  The TMA
     contains known positives and known negatives.
     I=  n  cases of t-cell or b-cell markers or cytokeratins.  25 to 30
     cases  i= s easy.  But when you are validated for more hard to find
     markers  (SV-= 40) then fewer cases is acceptable.  We always throw
     in  a slide that w= e know will not stain for sv-40 like a tonsil -
     then you can say it has spe= cificity.
     Any  inspector that I have come across is usually understanding= of
     this.  But I am sure that there are exceptions to this.........esp
ecially if they are not familiar with immunohistochemistry.
     Loralee McMahon, HTL (ASCP)
     Immunohistochemistry Supervisor
     Strong M= emorial Hospital
     Department of Surgical Pathology
     (585) 275-7210
          ______________________     5F__= _______________
     From:          histonet-bounces <@t> lis=          ts.utsouthwestern.edu
     [histonet-bounces <@t> lists.utsouthwestern.edu]     On    Behalf=    Of
     thisisann <@t> aol.com [thisisann <@t> aol.com]
     Sent: Wednesday, April 28, 201= 0 2:47 PM
     To: histonet <@t> lists.utsouthwestern.edu
     Subject: [Histonet] R= esponses to IHC CAP Validation question
     The following is one respone= I rec'd:
     1.   I  asked  CAP  who told me that they do not currentl= y have a
     guideline on
     validating but that they
     recommend what is in t= he following book:
     Quality  Management  In  Anatomic  Pathology,  Promoting  P= atient
     Safety
     Through Systems Improvement and Error
     by Raouf E. Nakhl= eh, MD & Patrick Fitzgibbons, MD editors
     sold by CAP !
     Chapter 8-= Quality Management in IHC
     That is what we follow.
     I. Get a new antib= ody and optimize it with your positive control.
     II.  Once  optimized  you  n= eed to run it on cases expected to be
     positive
     (how many?)
     "a suffien= t size ..."
     III. Must also be run on cases expected to be negative. (how= many?
     IV.  In a situation where you cannot expect a lot of cases or such
a
     case has
     never been presented in your lab, then you must say just = that.
     (ex. some of the hormones we just use a pituitary)
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References

   1. 3D"mailto:tonia.richmond <@t> gracepathology.com"
   2. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"
   3. 3D"http://=/
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