[Histonet] questions

Tony Henwood AnthonyH <@t> chw.edu.au
Tue Mar 30 19:21:58 CDT 2010


Apart from hoping the blocks of tissue are not too thick, we microwave
the cassettes in 10%NBF in a Milestone Mega TT - 2 hours at 45oC. This
ensures adequate fixation prior to processing on a Shandon Excelsior
Tissue Processor.


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Webb,
Dorothy L
Sent: Wednesday, 31 March 2010 6:04 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] questions

We have been having problems with underprocessed placental membrane,
some of which are cut fairly thick, but am seeing it on more than just
the thich samples.  Does anyone out there in histoland have a special
process for placentas or any helpful hints?  I do know that many times
the placentas sit without formalin in L&D for hours before they bring
them to histo and add formalin and this seems to me it could be a
factor, even though we have them sitting in formalin for a few hours
before processing.

Also, does anyone do the high-iron diamine stain for intestinal mucin
staining?  Do you do it with an Alcian Blue-PAS stain?

Thank you ahead of time for any and all responses!!

Dorothy Webb, HT (ASCP)
Regions Histology Lab

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