becky.garrison <@t> jax.ufl.edu
Tue Mar 30 15:49:42 CDT 2010
We receive the placentas fresh (they are refrigerated in L&D before
transport to Pathology); add lots of formalin (use 163 oz containers)
and let fix overnight. Early next morning, placenta is grossed and
cassettes sit in formalin til end of day. This formalin is changed at
least once so that bloody formalin is replaced with fresh. Placed on
processor at end of second day after receipt.
When we had L&D add formalin, there was never enough formalin added and
the placentas sat unfixed at room temperature for long periods of time.
This procedure works better for us. Yes, we can not meet the CAP
for 2 day TAT but do end up with a consistently better quality product
for this tissue type. (Placentas make up a small portion of overall
workload, so overall TAT is not affected). Prior to this procedure,
up a disproportionate amount of reprocessed blocks.
Jacksonville, FL 32209
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Webb,
Sent: Tuesday, March 30, 2010 3:04 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] questions
We have been having problems with underprocessed placental membrane,
some of which are cut fairly thick, but am seeing it on more than just
the thich samples. Does anyone out there in histoland have a special
process for placentas or any helpful hints? I do know that many times
the placentas sit without formalin in L&D for hours before they bring
them to histo and add formalin and this seems to me it could be a
factor, even though we have them sitting in formalin for a few hours
Also, does anyone do the high-iron diamine stain for intestinal mucin
staining? Do you do it with an Alcian Blue-PAS stain?
Thank you ahead of time for any and all responses!!
Dorothy Webb, HT (ASCP)
Regions Histology Lab
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