[Histonet] questions

[Webb, Dorothy L]

We have been having problems with underprocessed placental membrane, some of which are cut fairly thick, but am seeing it on more than just the thich samples.  Does anyone out there in histoland have a special process for placentas or any helpful hints?  I do know that many times the placentas sit without formalin in L&D for hours before they bring them to histo and add formalin and this seems to me it could be a factor, even though we have them sitting in formalin for a few hours before processing.

Also, does anyone do the high-iron diamine stain for intestinal mucin staining?  Do you do it with an Alcian Blue-PAS stain?

Thank you ahead of time for any and all responses!!

Dorothy Webb, HT (ASCP)
Regions Histology Lab



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