[Histonet] Alcian Blue 2.5

[Malika Benatti]

Hey Laurie,

Try with home made Alcian Blue Solution : pH 2.5 1g Alcian Blue in 100  
ml of 3% Acetic Acid  ( check pH adjust accordingly, filter AB  
solution before use) also rather than rinsing with large amount of  
water, blot dry section this should help alteration of staining, then  
rinse briefly before next step.

Hope this help

Malika

Malika Benatti

Specialist BMS
Camelia Botnar Laboratories
Histopathology Department
Great Ormond Street Hospital
London WC1N 3JH


Tel:  +44 20 7405 9200 ext 5475
Fax:  +44 20 7829 7875

benatm <@t> gosh.nhs.uk

  " ... Smile it confuses people ..."

On 24 Mar 2010, at 09:32, "Laurie Elmgren" <lelmgren <@t> sunriselab.com>  
wrote:

> Hi Everyone,
>
> Lately, we have been having a problem with our Alcian Blue. It is fine
> up to the washing step. Once it hits the water, the blue fades to
> unacceptable. We have tried reducing the water rinse after the blue to
> just a quick rinse in d-H20, and it is still poor. The vendor replaced
> the kit, and it worked fine the first time, then two weeks later, the
> same problem arose. Three different techs have tried the stain three
> times. Any suggestions?
>
>
>
> Laurie Elmgren
>
> Histology Supervisor
>
> Sunrise Medical Labs
>
> 240 Motor Pkwy
>
> Hauppauge, NY 11788
>
> (631)435-1515x1108
>
>
>
>
>
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