[Histonet] Alcian Blue 2.5

[Geoff McAuliffe]

The pH of the stain may be too high, it should be 2.5-2.6 so check it 
with a pH meter.
If the pH is fine you/your vendor may have a bad batch of dye since 
under normal conditions the stain is difficult to wash out.
Make sure the dye is certified by the Biological Stain Commission. If 
there is no certification tag the dye is not certified.

Geoff

Laurie Elmgren wrote:
> Hi Everyone,
>
> Lately, we have been having a problem with our Alcian Blue. It is fine
> up to the washing step. Once it hits the water, the blue fades to
> unacceptable. We have tried reducing the water rinse after the blue to
> just a quick rinse in d-H20, and it is still poor. The vendor replaced
> the kit, and it worked fine the first time, then two weeks later, the
> same problem arose. Three different techs have tried the stain three
> times. Any suggestions?
>
>  
>
> Laurie Elmgren
>
> Histology Supervisor
>
> Sunrise Medical Labs
>
> 240 Motor Pkwy
>
> Hauppauge, NY 11788
>
> (631)435-1515x1108
>
>  
>
>  
>
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-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff <@t> umdnj.edu
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