SPAM-LOW: RE: [Histonet] Incubation of Ab more then 1 hour in autostainer

[Patsy Ruegg]

Yea I don't do incubations for longer than one hour on the autostainer and I
do put thin trays of water under the slides and on the sides of the machine
(it is very dry in Colorado), but I can think of a way you could do longer
incubations if you were around, put a coverslip or parafilm over the section
after the ab has been applied, you would just have to be there to take it
off before the machine goes on to the next steps.

This is one area the Leica Bond with coverplates and the Ventana with liq
coverslip has over the Dako.  I do 2 hour hybridization at 37dc for ish on
the bond with the coverplates and the sections do not dry out.

Regards,

Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Liz Chlipala
Sent: Thursday, March 18, 2010 2:05 PM
To: Margaryan, Naira; histonet <@t> lists.utsouthwestern.edu
Subject: SPAM-LOW: RE: [Histonet] Incubation of Ab more then 1 hour in
autostainer

We have done up to an hour but not longer, if I were you I would have
two antibody steps and just to add some more reagent after an hour or
1.5 hours

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Margaryan, Naira
Sent: Thursday, March 18, 2010 1:39 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Incubation of Ab more then 1 hour in autostainer
Importance: High

Hi Histonetters!

I have a stupid question but I Have to ask.

Does anyone perform an Incubation of Ab that required more then 1 hour
(2-3 hours) in autostainer? Does autostainer keep slides wet or slides
sometimes are getting dry? 

I know that slides should not be dry in any step of IHC.

Thank you very much!

Naira

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