[Histonet] Incubation of Ab more then 1 hour in autostainer

Liz Chlipala liz <@t> premierlab.com
Thu Mar 18 15:04:47 CDT 2010

We have done up to an hour but not longer, if I were you I would have
two antibody steps and just to add some more reagent after an hour or
1.5 hours


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Margaryan, Naira
Sent: Thursday, March 18, 2010 1:39 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Incubation of Ab more then 1 hour in autostainer
Importance: High

Hi Histonetters!

I have a stupid question but I Have to ask.

Does anyone perform an Incubation of Ab that required more then 1 hour
(2-3 hours) in autostainer? Does autostainer keep slides wet or slides
sometimes are getting dry? 

I know that slides should not be dry in any step of IHC.

Thank you very much!


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