[Histonet] Incubation of Ab more then 1 hour in autostainer

[Kim Merriam]

Hi Naira,

We routinely do a 2 hour incubation in primary, but we make sure that more antibody is on the slide than would be necessary for most other reagents (500-600 ul).  We have not had any issues of drying.  If you are concerned about it, just make it 2 antibody steps instead of 1 on the machine.

Kim
 Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA 




________________________________
From: "Margaryan, Naira" <NMargaryan <@t> childrensmemorial.org>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Sent: Thu, March 18, 2010 3:38:33 PM
Subject: [Histonet] Incubation of Ab more then 1 hour in autostainer

Hi Histonetters!

I have a stupid question but I Have to ask.

Does anyone perform an Incubation of Ab that required more then 1 hour (2-3 hours) in autostainer? Does autostainer keep slides wet or slides sometimes are getting dry? 

I know that slides should not be dry in any step of IHC.

Thank you very much!

Naira

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