[Histonet] Incubation of Ab more then 1 hour in autostainer

Kim Merriam kmerriam2003 <@t> yahoo.com
Fri Mar 19 06:56:19 CDT 2010

Hi Naira,

We routinely do a 2 hour incubation in primary, but we make sure that more antibody is on the slide than would be necessary for most other reagents (500-600 ul).  We have not had any issues of drying.  If you are concerned about it, just make it 2 antibody steps instead of 1 on the machine.

 Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA 

From: "Margaryan, Naira" <NMargaryan <@t> childrensmemorial.org>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Sent: Thu, March 18, 2010 3:38:33 PM
Subject: [Histonet] Incubation of Ab more then 1 hour in autostainer

Hi Histonetters!

I have a stupid question but I Have to ask.

Does anyone perform an Incubation of Ab that required more then 1 hour (2-3 hours) in autostainer? Does autostainer keep slides wet or slides sometimes are getting dry? 

I know that slides should not be dry in any step of IHC.

Thank you very much!


Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu


More information about the Histonet mailing list