[Histonet] Incubation of Ab more then 1 hour in autostainer

Andrea T. Hooper andreahooper <@t> rocketmail.com
Mon Mar 22 08:28:06 CDT 2010

We used to do overnight in our DAKO Autostainer many years ago, and we kept many containers of dH20 in the incubator to make a humid chamber. Seemed to work well - although we realized overnight was overkill and 1-2 h was sufficient. In fact, given this we started to always put humid chambers inside for all incubations, short or long, as even after 1h we noticed some edge drying on the sections. It improved the quality and reliability of the sections.

--- On Thu, 3/18/10, Margaryan, Naira <NMargaryan <@t> childrensmemorial.org> wrote:

From: Margaryan, Naira <NMargaryan <@t> childrensmemorial.org>
Subject: [Histonet] Incubation of Ab more then 1 hour in autostainer
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Thursday, March 18, 2010, 7:38 PM

Hi Histonetters!

I have a stupid question but I Have to ask.

Does anyone perform an Incubation of Ab that required more then 1 hour (2-3 hours) in autostainer? Does autostainer keep slides wet or slides sometimes are getting dry? 

I know that slides should not be dry in any step of IHC.

Thank you very much!


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