[Histonet] tb-controls selfmade

Anthony Reilly Tony_Reilly <@t> health.qld.gov.au
Mon Mar 15 18:32:29 CDT 2010

Hi Gudren
I have not done this with TB but I have with other bacteria to produce controls for gram stains.  You need to have the tissue in a nutrient broth appropriate for the organism.  You need to consult with a laboratory that specialises in Mycobacteria to find the appropriate medium to use.  Also what may be relevant is that in the laboratory it takes up to six weeks to culture TB.  Not sure but this may be why you are not getting results.  

Tony Reilly
Chief Scientist, Anatomical Pathology
Pathology Queensland-PA Laboratory
Clinical and Statewide Services Division| QueenslandHealth
Level 1, Building 15,Princess Alexandra Hospital
Ipswich Road,WOOLLOONGABBA  Qld4102
Ph: 07 3176 2412
Mob: 0402 139411
Fax: 07 3176 2930
Email: tony_reilly <@t> health.qld.gov.au
Web:  www.health.qld.gov.au/qhcss/

>>> "Gudrun Lang" <gu.lang <@t> gmx.at> 16/03/2010 1:14 am >>>
Hi all!

Can someone provide me with a procedure for selfmade Tb-control for

I tried incubating some lungtissue (2x2x2 cm) in a solution with 3-6 drops
of bacteria-suspension for 3 hours and for 18 hours. Afterwards fixing in
NBF for 4 days.

I had no success. No stainable mycobacteria were found.



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