Hi all! Can someone provide me with a procedure for selfmade Tb-control for ZN-staining. I tried incubating some lungtissue (2x2x2 cm) in a solution with 3-6 drops of bacteria-suspension for 3 hours and for 18 hours. Afterwards fixing in NBF for 4 days. I had no success. No stainable mycobacteria were found. Bye Gudrun