[Histonet] spinal cord paraffin embedding

[V. Neubert]

Hi,

I processed small porcine spinal cord once (diameter ~3-5mm).
My pathologist did not mention any abnormalities, so I guess our
standard protocol for every type of tissue was fine.

for 1h each:
NBF
70% isopropanol ( = 2-propanol)
80% isopropanol
95% isopropanol
100% isopropanol
100% isopropanol
100% isopropanol
xylene
xylene
paraffine
paraffine

If nothing else helps, maybe you want to give that a try with slightly
shorter times in the alcoholic solutions, as your specimen seems to be
smaller in size.


>    Dear all,
>    I'm quite new in morphological analysis and Histology.
>    In  my  l=ab we are processing rat spinal cord to perform luxol fast
>    blue staining.
>    First   we   fix   the   spinal  cord  (only  lumbar  portion)  in  4%
>    parafolmaldehyde  (4h) =y immesion, then the samples are embedded in
>    paraffin  using  a authomatic Leica ASP300 machine. When observed, the
>    white  matter  of the spinal cord is full of holes and myelin seems to
>    be absent.
>    I  guess the problem was related to the processing steps=erformed by
>    the machine, in particular the Xylol step.
>    Is  there someone who can help me sharing his/her experience on spinal
>    cord process=ng protocol?
>    Any suggestion will be highly appreciated.
>    Regards
>    -- 
>    Dott.ssa Elisa Ballarini,PhD student
>    Dipartimento di Neuroscie=ze e Tecnologie Biomediche
>    Università degli Studi Milano-Bicocca
>    Via C=dore 48-20052,Monza,MB
>    e.ballarini1 <@t> campus.unimib.it
>    Tel. 02-64488119
>    Fax. 02-64488253
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>   


Am 15.03.2010 15:05, schrieb Elisa Ballarini:
>    Dear all,
>    I'm quite new in morphological analysis and Histology.
>    In  my  l=ab we are processing rat spinal cord to perform luxol fast
>    blue staining.
>    First   we   fix   the   spinal  cord  (only  lumbar  portion)  in  4%
>    parafolmaldehyde  (4h) =y immesion, then the samples are embedded in
>    paraffin  using  a authomatic Leica ASP300 machine. When observed, the
>    white  matter  of the spinal cord is full of holes and myelin seems to
>    be absent.
>    I  guess the problem was related to the processing steps=erformed by
>    the machine, in particular the Xylol step.
>    Is  there someone who can help me sharing his/her experience on spinal
>    cord process=ng protocol?
>    Any suggestion will be highly appreciated.
>    Regards
>    -- 
>    Dott.ssa Elisa Ballarini,PhD student
>    Dipartimento di Neuroscie=ze e Tecnologie Biomediche
>    Università degli Studi Milano-Bicocca
>    Via C=dore 48-20052,Monza,MB
>    e.ballarini1 <@t> campus.unimib.it
>    Tel. 02-64488119
>    Fax. 02-64488253
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>