[Histonet] spinal cord paraffin embedding
e.ballarini1 <@t> campus.unimib.it
Mon Mar 15 09:05:50 CDT 2010
I'm quite new in morphological analysis and Histology.
In my l blue staining.
First we fix the spinal cord (only lumbar portion) in 4%
parafolmaldehyde (4h) paraffin using a authomatic Leica ASP300 machine. When observed, the
white matter of the spinal cord is full of holes and myelin seems to
I guess the problem was related to the processing steps the machine, in particular the Xylol step.
Is there someone who can help me sharing his/her experience on spinal
cord process Any suggestion will be highly appreciated.
Dott.ssa Elisa Ballarini,PhD student
Dipartimento di Neuroscie Università degli Studi Milano-Bicocca
Via C e.ballarini1 <@t> campus.unimib.it
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