SPAM-LOW: RE: [Histonet] Red Chromogen for MITF antibody

Patsy Ruegg pruegg <@t>
Sat Mar 13 10:02:48 CST 2010

Is the Perma Red for hrp?  If you switch to an alk.phos detection system you
could use fast red which is much redder than AEC.  Dako sells ap/red
detection systems, but I prefer to use Vector Red for AP.


Patsy Ruegg, HT(ASCP)QIHC
12635 Montview Blvd. Ste.215
Aurora, CO 80045
fax 720-859-4110

-----Original Message-----
From: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] On Behalf Of McMahon,
Loralee A
Sent: Friday, March 12, 2010 6:23 AM
To: Gareth Blaeuer Davis; histonet <@t>
Subject: SPAM-LOW: RE: [Histonet] Red Chromogen for MITF antibody

We use Diagnostic Biosystems Perma Red with the Dako system.
Works very well

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From: histonet-bounces <@t>
[histonet-bounces <@t>] On Behalf Of Gareth Blaeuer
Davis [gareth.davis <@t>]
Sent: Thursday, March 11, 2010 7:32 PM
To: histonet <@t>
Subject: [Histonet] Red Chromogen for MITF antibody


Does anyone do MITF immunostains with a Red Chromogen.  We are using a Dako
autostainer, and in the past have used AEC chromogen on MITF.  Our
pathologist doesn't like it, because it doesn't get red enough.  We would
like something we could use with current protocols on the Dako, but can't
find anything.  Any suggestions?

Gareth Blaeuer Davis, HT, BS.

Pathology Associates of St. Thomas

Nashville, Tn 37205


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