SPAM-LOW: RE: [Histonet] Red Chromogen for MITF antibody

[McMahon, Loralee A]

The perma red is for HRP or alk phos. Depends on your detection polymer.    

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: Patsy Ruegg [pruegg <@t> ihctech.net]
Sent: Saturday, March 13, 2010 11:02 AM
To: McMahon, Loralee A; 'Gareth Blaeuer Davis'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: SPAM-LOW:  RE: [Histonet] Red Chromogen for MITF antibody

Is the Perma Red for hrp?  If you switch to an alk.phos detection system you
could use fast red which is much redder than AEC.  Dako sells ap/red
detection systems, but I prefer to use Vector Red for AP.

Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net
www.ihcrg.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of McMahon,
Loralee A
Sent: Friday, March 12, 2010 6:23 AM
To: Gareth Blaeuer Davis; histonet <@t> lists.utsouthwestern.edu
Subject: SPAM-LOW: RE: [Histonet] Red Chromogen for MITF antibody

We use Diagnostic Biosystems Perma Red with the Dako system.
www.dbiosys.com
Works very well


Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu
[histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gareth Blaeuer
Davis [gareth.davis <@t> hotmail.com]
Sent: Thursday, March 11, 2010 7:32 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Red Chromogen for MITF antibody

Hi,

Does anyone do MITF immunostains with a Red Chromogen.  We are using a Dako
autostainer, and in the past have used AEC chromogen on MITF.  Our
pathologist doesn't like it, because it doesn't get red enough.  We would
like something we could use with current protocols on the Dako, but can't
find anything.  Any suggestions?

Gareth Blaeuer Davis, HT, BS.

Pathology Associates of St. Thomas

Nashville, Tn 37205

615-298-4100

_________________________________________________________________
Hotmail: Free, trusted and rich email service.
http://clk.atdmt.com/GBL/go/201469228/direct/01/____________________________
___________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet