[Histonet] Masson's Trichrome, Weigert Hematoxylin Equivalent

[Tony Henwood]

Colin,

Rather than Weigert's Hx you can use the Celestin Blue Harris Hx
sequence.

Stain 5minutes in Celestine Blue (0.5% Celestine Blue (CI 51050) in 5%
iron alum (ferric ammonium sulphate)), followed by Harris Hx 5 minutes,
contine Masson Staining. See:

Henwood T, Llewellyn B, Montgomery I, Nader A, Rittman B R (2007)
"Celestine blue and hemalum for staining nuclei" Biotechnic &
Histochemistry 82(3): 167-168.


Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
cscampbe <@t> uci.edu
Sent: Thursday, 11 March 2010 11:21 AM
To: HistoNet
Subject: [Histonet] Masson's Trichrome, Weigert Hematoxylin Equivalent


Hi Histonet,

I am performing the trichrome stain on cardiac tissue. The procedure
calls for Weigert's iron hematoxylin stain or equivalent. Would Harris
hematoxylin work as well. It's what I have readily available at the lab.

The instructions for preparing the Weigert stain involves mixing fresh
solutions from two stock solutions - but the staining process of all the
slides will take weeks. How long does the solution stay "fresh?"

Thanks!
-Colin


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