[Histonet] Masson's Trichrome, Weigert Hematoxylin Equivalent
cscampbe <@t> uci.edu
cscampbe <@t> uci.edu
Wed Mar 10 18:20:41 CST 2010
Hi Histonet,
I am performing the trichrome stain on cardiac tissue. The procedure calls
for Weigert's iron hematoxylin stain or equivalent. Would Harris
hematoxylin work as well. It's what I have readily available at the lab.
The instructions for preparing the Weigert stain involves mixing fresh
solutions from two stock solutions - but the staining process of all the
slides will take weeks. How long does the solution stay "fresh?"
Thanks!
-Colin
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