[Histonet] Masson's Trichrome, Weigert Hematoxylin Equivalent

John Kiernan jkiernan <@t> uwo.ca
Wed Mar 10 20:52:11 CST 2010

No. The PTA or PMA used in Masson's method is too acidic and would remove Harris's or any other haemalum. An iron-haematoxylin such as Weigert's or Lillie's gives an acid-resistant nuclear stain that is progressive and almost black. 
John Kiernan
Anatomy, UWO
London, Canada
= = =
----- Original Message -----
From: cscampbe <@t> uci.edu
Date: Wednesday, March 10, 2010 19:21
Subject: [Histonet] Masson's Trichrome, Weigert Hematoxylin Equivalent
To: HistoNet <histonet <@t> lists.utsouthwestern.edu>

> Hi Histonet,
> I am performing the trichrome stain on cardiac tissue. The 
> procedure calls
> for Weigert's iron hematoxylin stain or equivalent. Would Harris
> hematoxylin work as well. It's what I have readily available at 
> the lab.
> The instructions for preparing the Weigert stain involves mixing fresh
> solutions from two stock solutions - but the staining process of 
> all the
> slides will take weeks. How long does the solution stay "fresh?"
> Thanks!
> -Colin
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