[Histonet] Microwave processing and IHCs

[WILLIAM DESALVO]

I have used the Sakura Xpress for 5+ years and have had great success w/ all staining, w/ little to no changes. The Xpress does not use Xylene and that was a change from our conventional processing and reagents, so we did have to collect new control tissues. I say that anytime you change the process, you MUST collect new control tissue and you will ned to document. When CAP comes to visit and they see a MW processor, you will need to show them your validation and control tissue documentation. If you have the possibility of using both conventional or Microwave processd tissue, then make a modified sausage block to include both types of processing protocols, so that work can flow through the lab smoothly.

   
William DeSalvo, B.S., HTL(ASCP)

 
> From: greenjumpyone <@t> hotmail.com
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Mon, 1 Mar 2010 17:15:23 -0800
> Subject: [Histonet] Microwave processing and IHCs
> 
> 
> Does anyone here have any experience with using microwave tissue processing and then performing IHC stains on those same tissues?
> 
> We have new microwave processors and are trying to determine if we will need to get all new controls for our IHCs that have been microwave processed. If yes, we will need to do validations on the controls, but I am wondering if the MW will have any discernible effect on the reactivity of the tissue.
> 
> thanks for any help!
> 
> Michelle
> 
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