[Histonet] Help Resurrecting Dried Fixed Tissue

[Tony Henwood]

We have success with Sandison Method, see below:


Sandison Method

Solutions:
95% ethanol			300ml
38% Formalin		10ml
Sodium carbonate		10g
Water				690ml

Method:

Leave tissues in solution overnight or until they become soft. Replace
one third of fluid with 95% ethanol, repeat until tissue becomes firm.


Reference:

	"The histological examination of Mummified Tissue" Stain Techn
30:277-283, 1955

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Parsons,
Catherine
Sent: Thursday, 24 June 2010 1:16 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Help Resurrecting Dried Fixed Tissue


I am grasping at straws and hoping for ideas to salvage some formalin
fixed tissue specimens.  I work in a research lab, and am quite new to
histology and this particular lab.  A former student left formalin fixed
tissue specimens that were stored in 70% ethanol.  Unfortunately, the
seal failed on a few of the vials, the ethanol evaporated, and the
tissue is a dried out disaster at the bottom of the vial.

Has anyone has any experience / luck rehydrating tissues?  Really, any
information that I could possibly pull out of these specimens would be
welcome.

Cathy Parsons


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