[Histonet] X-gal staining troubleshooting

[Johnson, Teri]

Hi all,

I have a question about whole mount x-gal staining. Do any of you have experience with your samples turning brown after incubation with the x-gal?  If yes, do you know what causes this and how to keep it from happening?

Thanks in advance,

Teri Johnson, HT(ASCP)QIHC
Managing Director, Histology Facility
Stowers Institute for Medical Research
Kansas City, MO