[Histonet] tumour,lymph node and prostate preservation for histology

[Tony Henwood]

Carolina,

It will depend on what you want to do to the tissue after removal. For
Histology, placing the tissue in 10% phosphate buffered formalin is good
for morphology, special staains, immunohistochemistry and in situ
hybridisation. I have been able to extract RNA and DNA from formalin
fixed, paraffin embedded tissues that have been stored at room
temperature for 40 years! (and others have also shown this).

Contact me for further details.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Carolina
Soekmadji
Sent: Tuesday, 22 June 2010 4:35 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] tumour,lymph node and prostate preservation for
histology



Hi,

I am new to histology and have some very basic questions. I hope
somebody out there willing to help me.. I want to isolate tumours from
mice and preserve it for histology . I heard that there is different
method on how best preserving different organs/ tissue. Could any of you
please advise me a  method on how to best preserve tumour ? Also if
there a method  to preserve lymph node and prostate? Thank you very
much.

Best regards,
Carolina

Carolina Soekmadji
Postdoctoral Research Fellow
Australian Prostate Cancer Research Centre - Queensland | Institute of
Health and Biomedical Innovation |
t: 07 3176 7428 (APCRC - Princess Alexandra Hospital)
t: 07 3138 6286 (IHBI - QUT Kelvin Grove)
mobile: +61 423 111 807
f: 07 3176 7440
e: carolina.soekmadji <@t> qut.edu.au<mailto:carolina.soekmadji <@t> qut.edu.au>


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