[Histonet] how to prevent foldings on femoral head cartilage tissue

[Liz Chlipala]

Sometimes placing them on a hot plate at about 60C will help get out the
folds, the paraffin needs to melt and the sections need to turn clear
then take it off the hot plate.  If you leave it too long on the
hotplate the articular cartilage may fold over on itself.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Reuel
Cornelia
Sent: Thursday, June 17, 2010 11:10 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] how to prevent foldings on femoral head cartilage
tissue

Hello histonetters especailly hard tissue group
I have a  pig femoral head bone tissue embedded in paraffin and I have a
hard time getting rid of the folding problem. I tried to remedy by
lowering my temperature to 38 C and putting them in 5% alcohol before
placing them in water bath I still have a lots of folding formation on
some areas of the cartilage. Is there any other technique to remedy this
problem. I appreciate your help. Thank you.

reuel



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