[Histonet] Sakura Film Removal

Mighnon Lashus MLashus <@t> pathgroup.com
Thu Jul 29 14:43:13 CDT 2010

We 1st use straight acetone and leave the slide in anywhere from 1 to 3 minutes, then 20 seconds in 50/50 acetone/xylene, then dip in straight xylene.  Depending on how long the slide has been coverslipped, the times will need to be adjusted.

Mighnon Lashus, HT (ASCP)
PathGroup Lab
4071 S. Access Road, Suite 107
Chattanooga, TN  37406
423-493-0208 fax
mlashus <@t> pathgroup.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sharon Scalise
Sent: Thursday, July 29, 2010 3:37 PM
To: Paula Lucas; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Sakura Film Removal

We use a 50-50 solution of xylene and acetone.  Depending on how long the coverslips have been on, it takes from 20 minutes to an hour for them to come off.  If you are not decolorizing and re-staining, don't leave them too long in the solution as the stain will start to come out.

Sharon E. Scalise, HTL (ASCP)
Histology Supervisor
William Beaumont Hospital
Royal Oak, MI 48073
248 898-5981
sscalise <@t> beaumonthospitals.com

>>> "Paula Lucas" <plucas <@t> biopath.org> 7/29/2010 3:17 PM >>>
Hello all -

Is there a more effective and faster way to remove the film from the slide?
I had to decolorize a couple of H&E slides to do special stains, and it took
5 days for the film to remove. The slides were only a few days old and I
soaked it in fresh xylene.  I almost removed it too soon because the section
was starting to lift off with the film.

I appreciate the help

Paula Lucas

Lab Manager

Bio-Path Medical Group


Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

Important Notice:  This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential.  If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited.  If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255.  Thank you

More information about the Histonet mailing list