[Histonet] Sakura Film Removal

[Mighnon Lashus]

We 1st use straight acetone and leave the slide in anywhere from 1 to 3 minutes, then 20 seconds in 50/50 acetone/xylene, then dip in straight xylene.  Depending on how long the slide has been coverslipped, the times will need to be adjusted.

Mighnon Lashus, HT (ASCP)
PathGroup Lab
4071 S. Access Road, Suite 107
Chattanooga, TN  37406
423-493-0207
423-493-0208 fax
mlashus <@t> pathgroup.com



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sharon Scalise
Sent: Thursday, July 29, 2010 3:37 PM
To: Paula Lucas; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Sakura Film Removal

We use a 50-50 solution of xylene and acetone.  Depending on how long the coverslips have been on, it takes from 20 minutes to an hour for them to come off.  If you are not decolorizing and re-staining, don't leave them too long in the solution as the stain will start to come out.

Sharon E. Scalise, HTL (ASCP)
Histology Supervisor
William Beaumont Hospital
Royal Oak, MI 48073
248 898-5981
sscalise <@t> beaumonthospitals.com


>>> "Paula Lucas" <plucas <@t> biopath.org> 7/29/2010 3:17 PM >>>
Hello all -



Is there a more effective and faster way to remove the film from the slide?
I had to decolorize a couple of H&E slides to do special stains, and it took
5 days for the film to remove. The slides were only a few days old and I
soaked it in fresh xylene.  I almost removed it too soon because the section
was starting to lift off with the film.



I appreciate the help

Paula Lucas

Lab Manager

Bio-Path Medical Group

FV, CA

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