[Histonet] Sakura Film Removal

annigyg <@t> gmail.com annigyg <@t> gmail.com
Thu Jul 29 14:19:23 CDT 2010

The slips will not come off with xylene
Soak in acetone for 5mins
The slip swells and falls off
Without letting the slide dry or turn milky white, dip into xylene a few times
If milkiness appears, dip in clean acetone a few times and back to xylene
Recoverslip as and when needed
Simple really

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-----Original Message-----
From: "Paula Lucas" <plucas <@t> biopath.org>
Sender: histonet-bounces <@t> lists.utsouthwestern.edu
Date: Thu, 29 Jul 2010 12:17:54 
To: <histonet <@t> lists.utsouthwestern.edu>
Subject: [Histonet] Sakura Film Removal

Hello all - 


Is there a more effective and faster way to remove the film from the slide?
I had to decolorize a couple of H&E slides to do special stains, and it took
5 days for the film to remove. The slides were only a few days old and I
soaked it in fresh xylene.  I almost removed it too soon because the section
was starting to lift off with the film.


I appreciate the help

Paula Lucas

Lab Manager

Bio-Path Medical Group


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