[Histonet] Sakura Film Removal

Jason McGough jmcgough <@t> clinlab.com
Thu Jul 29 14:18:52 CDT 2010

We soak the slides in acetone for 5-10 minutes and the film will peel off
very easily.

Jason McGough HT(ASCP)
Account Representative - Anatomic Pathology
Clinical Laboratory of the Black Hills
2805 5th Street Suite 210
Rapid City, SD 57701
605-343-2267 Ext 127
605-718-3779 (Fax)
jmcgough <@t> clinlab.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Paula
Sent: Thursday, July 29, 2010 1:18 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Sakura Film Removal

Hello all -

Is there a more effective and faster way to remove the film from the slide?
I had to decolorize a couple of H&E slides to do special stains, and it took
5 days for the film to remove. The slides were only a few days old and I
soaked it in fresh xylene.  I almost removed it too soon because the section
was starting to lift off with the film.

I appreciate the help

Paula Lucas

Lab Manager

Bio-Path Medical Group


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