[Histonet] Gordon and Sweets retic stain

[Johnson, Nacaela]

Hello! 

I am having a problem with my tissue washing off of the slides during
the retic stain. The majority of the tissue is falling off during the
Working silver solution step because of the alkalinity of the solution.
The tissue is bone marrow and I use Halt in my water bath. My thoughts
are to add a mild acid to the working solution to bring the pH down, but
I am not sure of what the effects are on the solution. I do not want the
silver to not impregnate. Has anyone had this problem before and found a
solution? 

 
 
Thanks,
 
Nacaela Johnson
Histology Technician
KCCC Pathology
12000 110th St., Ste. 400
Overland Park, KS 66210
Office:  913-234-0576
Fax:  913-433-7639
Email:  Nacaela.Johnson <@t> USOncology.com
 
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