[Histonet] Brain Problems

[sgoebel <@t> xbiotech.com]

   Try  cooling down your water bath with ice before you cut the brai   All  CNS  tissue  will  act like you described.  You may end up w   more  wrinkles  with the cooler bath, but put the slides in a 70C oven
   fo
   Happy Cuttin
   Sarah Goebel, B.A., HT (ASCP)

   Histotechnician
   
   XBiotech USA Inc.

   8201 East Riverside Dr. Bldg 4 Suite 10
   Austin, Texas  78744

   (512)386-5107

   -------- Original Message --------
   Subject: [Histonet] Brain Problems
   From: "Poole, Kimberly" <[1]Kimberly.Poole <@t> drdc-rddc.gc.ca>
   Date: Tue, July 27, 2010 7:27 am
   To: <[2]histonet <@t> lis   Hi there,
   I  am  currently  using  rat  brain for histology but something is not
   working.    Station
   Solution
   Routine (mins)
   1
   Formalin
   10
   2
   Formalin
   10
   3
   70% Ethanol
   60
   4
   80% Ethanol
   240
   5
   96% Ethanol
   240
   6
   100% Ethanol
   240
   7
   Xylene
   120
   8
   Xylene
   120
   9
   Xylene
   120
   10
   Paraffin Wax
   120
   11
   Paraffin Wax
   180
   12
   Paraffin Wax
   180
   TOTAL TIME
   27 hours 20 minutes
   I  embedded  my  brains  and  then cut them afterwards. When the brain
   section  h   Then  it  spreads  re   paraffin  the  brain  bursts  open     something  wrong  in  the processing stage? Is    too much. Any feedback would be greatly appreciated.<   Kimberly Poole [3]B.Sc
   Casualty Management Section | Section de la gestion des blessés
   Defence  Research  and  Development  Canada  Suffield  |  Recherche et
   dévelo   Medicine Hat, AB, Canada T1A 8K6
   [4]kimberly.poole <@t> drdc-rdd   mberly.poole <@t> drdc-rddc.gc.ca>
   Telephone   |   Téléphone   403-544-5347   /   Facsimile   |
   Téléco   Government of Canada | Gouvernement du Canada
   _______________________________________________
   Histonet mailing list
   [6]Histonet <@t> lists.utsou   [7]http:
References

   1. 3D"mailto://Kimberly.Poole@drdc-rddc.gc=/
   2. 3D"mailto://histonet@lists.utsouthwestern.edu"/
   3. 3D"http://B.Sc"/
   4. 3D"mailto://kimberly.poole@drdc-rddc.gc.ca"/
   5. 3D"mailto:kimberly.poole <@t> drdc-rddc.gc.ca"
   6. 3D"mailto://Histonet@lists.utsouthwestern.edu"/
   7. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"