[Histonet] Re: [Sectioning] Brain Tissue

[Sherwood, Margaret]

To All:
 
We have been experiencing problems sectioning mouse brain.  The sectioning is
fine, but we have problems in the water bath.  At 45 degrees C (peel-a-way
paraffin-Polysciences), the sections break apart but we don't get wrinkles.
With paraplast extra, we use 36 degrees C, but the sections are wrinkled and
disintegrate.   
 
I would appreciate hearing from listers as to what you do:  what temperature is
your water bath, what embedding media do you use, etc?  Any help would be
appreciated!
 
Thanks!
Peggy
 
Peggy Sherwood
Lab Associate, Photopathology 
Wellman Center for Photomedicine (EDW 214)
Massachusetts General Hospital
55 Fruit Street
Boston, Massachusetts 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood <@t> partners.org
 


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