[Histonet] RE: Histonet Digest, Vol 80, Issue 30
prashant sareen
sareenprashant <@t> hotmail.com
Mon Jul 26 13:38:07 CDT 2010
Try reducing the processing time and try very fine cutting and I think the microchatter will go.
Thanks
Prashant Sareen BS , M.B.A , HT (ASCP)
Associate Scientist III
Comprehensive Animal health Services
Bioreliance Corporation
14930 Broschart Road,
Rockville, MD 20850
Office: 301-610-2744
Histology: 301-610-2997
Cell: 240-315-5541
Email: prashant.sareen <@t> bioreliance.com
> From: histonet-request <@t> lists.utsouthwestern.edu
> Subject: Histonet Digest, Vol 80, Issue 30
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Mon, 26 Jul 2010 10:02:53 -0700
>
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> Today's Topics:
>
> 1. Re: Artifacts in histology section (Joseph Saby)
> 2. Re water problem (Steven Weston)
> 3. RE: Re water problem (Goins, Tresa)
> 4. Re: Artifacts in histology section (Kim.Donadio <@t> bhcpns.org)
> 5. Special Stain Storage (kristen arvidson)
> 6. RE: Special Stain Storage (Podawiltz, Thomas)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Sun, 25 Jul 2010 10:49:05 -0700 (PDT)
> From: Joseph Saby <saby_joseph_a <@t> yahoo.com>
> Subject: Re: [Histonet] Artifacts in histology section
> To: Aazath Raj <aazath <@t> hotmail.com>, histonet <@t> lists.utsouthwestern.edu
> Message-ID: <431113.5115.qm <@t> web114420.mail.gq1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> What you are describing might be microchatter. These will be sharp parallel
> lines/cracks that run parallel to the knife edge and are only visible under the
> microscope.
>
> The usuall cause is a combination of overprocessing and rough facing that is too
> aggressive and/or with too dull a blade. Overprocessing makes the tissue very
> hard and somewhat brittle. The thick sections/dull knife cause the tissue to
> compress and then release, causing the chatter. The actual danage is in the
> block face.
>
> Once you have the problem in a block, if the tissue is thick enough, you might
> be able to repeatedly soak the block in ice water and gently (with a fairly
> sharp knife) reface. With luck, you might be able to get through the damaged
> block face.
>
>
> Another artifact I have seen is similar, but the chatter appears very blurry.
> This is usually caused be poor fixation/processing, then oversoaking the blocks
> after facing. The trick here is to reface the block, then chill it without
> exposure to water. I've sectioned such blocks after placing them in a freezer
> to chill them thoroughly. This will help to obtain a section, but may not fix
> the staining problems that might show up later.
>
>
> Good luck!
>
> Joe Saby, BA HT
>
>
>
>
> ________________________________
> From: Aazath Raj <aazath <@t> hotmail.com>
> To: histonet <@t> lists.utsouthwestern.edu
> Sent: Fri, July 23, 2010 11:26:28 AM
> Subject: [Histonet] Artifacts in histology section
>
>
>
> Dear Friends,
>
> I am an Histology Technologist. I am having a problem here,while
> sectioning am not seeing and scoring artifacts on the section but in the
> microscope am seeing a tearing artifacts particularly in endoscopy biopsies. am
> not able to locate where is the problem,is that because of blades or due to
> micro-crystallization of wax or due to any processing problem. Its not
> consistently in all but i get it on some blocks every day. Can any one help me
> in sorting it out. If anybody is interested in will send the picture of those
> section.
>
>
>
>
>
> with regards,
>
> Aazathraj.P
>
> Technical Officer,
>
> Apollo Hospitals-chennai
>
> India.
>
> aazath <@t> hotmail.com
>
>
>
> _________________________________________________________________
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>
> ------------------------------
>
> Message: 2
> Date: Mon, 26 Jul 2010 13:49:31 +1000
> From: Steven Weston <Steven.Weston <@t> utas.edu.au>
> Subject: [Histonet] Re water problem
> To: histonet list <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <C87342EB.2535%steven.weston <@t> utas.edu.au>
> Content-Type: text/plain; charset="iso-8859-1"
>
> We had this problem when we started using APTS coated slides and left out adhesive from the water bath. A simple solution I have found is to add a single drop of triton x100 (or similar detergent) to my full water bath and mix before heating the water. This reduces the surface tension of the water and allows it to run off the slide.
> Regards
> Steve weston
> Menzies Research Institute
> Hobart Tasmania, Australia
>
>
> ------------------------------
>
> Message: 3
> Date: Mon, 26 Jul 2010 15:39:05 +0000
> From: "Goins, Tresa" <TGoins <@t> mt.gov>
> Subject: [Histonet] RE: Re water problem
> To: Steven Weston <Steven.Weston <@t> utas.edu.au>
> Cc: "Histonet <@t> lists.utsouthwestern.edu"
> <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <CA4DF32ED505D94BB55E95487D8E98410497BD <@t> DOAISD5205.state.mt.ads>
> Content-Type: text/plain; charset="us-ascii"
>
> Thanks for the great tip Steve - it works great!
>
>
> Tresa Goins
> Veterinary Diagnostic Lab
> Department of Livestock
> Bozeman, Montana
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Steven Weston
> Sent: Sunday, July 25, 2010 9:50 PM
> To: histonet list
> Subject: [Histonet] Re water problem
>
> We had this problem when we started using APTS coated slides and left out adhesive from the water bath. A simple solution I have found is to add a single drop of triton x100 (or similar detergent) to my full water bath and mix before heating the water. This reduces the surface tension of the water and allows it to run off the slide.
> Regards
> Steve weston
> Menzies Research Institute
> Hobart Tasmania, Australia
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Mon, 26 Jul 2010 10:57:58 -0500
> From: Kim.Donadio <@t> bhcpns.org
> Subject: Re: [Histonet] Artifacts in histology section
> To: Joseph Saby <saby_joseph_a <@t> yahoo.com>
> Cc: histonet <@t> lists.utsouthwestern.edu, Aazath Raj
> <aazath <@t> hotmail.com>, histonet-bounces <@t> lists.utsouthwestern.edu
> Message-ID:
> <OFF4B67742.F26D1A0C-ON8625776C.0057150C-8625776C.0057B44E <@t> bhcpns.org>
> Content-Type: text/plain; charset="US-ASCII"
>
> It's very difficult to diagnosis a problem such as this without hands on.
> But one thing that I noticed years ago that was so simple could cause
> this. A build up of tissues on the back of the blade holder. Worth a look.
>
>
>
>
>
> Kim Donadio
> Pathology Supervisor
> Baptist Hospital
> 1000 W Moreno St.
> Pensacola FL 32501
> Phone (850) 469-7718
> Fax (850) 434-4996
>
>
>
> Joseph Saby <saby_joseph_a <@t> yahoo.com>
> Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
> 07/25/2010 12:49 PM
>
> To
> Aazath Raj <aazath <@t> hotmail.com>, histonet <@t> lists.utsouthwestern.edu
> cc
>
> Subject
> Re: [Histonet] Artifacts in histology section
>
>
>
>
>
>
> What you are describing might be microchatter. These will be sharp
> parallel
> lines/cracks that run parallel to the knife edge and are only visible
> under the
> microscope.
>
> The usuall cause is a combination of overprocessing and rough facing that
> is too
> aggressive and/or with too dull a blade. Overprocessing makes the tissue
> very
> hard and somewhat brittle. The thick sections/dull knife cause the tissue
> to
> compress and then release, causing the chatter. The actual danage is in
> the
> block face.
>
> Once you have the problem in a block, if the tissue is thick enough, you
> might
> be able to repeatedly soak the block in ice water and gently (with a
> fairly
> sharp knife) reface. With luck, you might be able to get through the
> damaged
> block face.
>
>
> Another artifact I have seen is similar, but the chatter appears very
> blurry.
> This is usually caused be poor fixation/processing, then oversoaking the
> blocks
> after facing. The trick here is to reface the block, then chill it
> without
> exposure to water. I've sectioned such blocks after placing them in a
> freezer
> to chill them thoroughly. This will help to obtain a section, but may not
> fix
> the staining problems that might show up later.
>
>
> Good luck!
>
> Joe Saby, BA HT
>
>
>
>
> ________________________________
> From: Aazath Raj <aazath <@t> hotmail.com>
> To: histonet <@t> lists.utsouthwestern.edu
> Sent: Fri, July 23, 2010 11:26:28 AM
> Subject: [Histonet] Artifacts in histology section
>
>
>
> Dear Friends,
>
> I am an Histology Technologist. I am having a problem
> here,while
> sectioning am not seeing and scoring artifacts on the section but in the
> microscope am seeing a tearing artifacts particularly in endoscopy
> biopsies. am
> not able to locate where is the problem,is that because of blades or due
> to
> micro-crystallization of wax or due to any processing problem. Its not
> consistently in all but i get it on some blocks every day. Can any one
> help me
> in sorting it out. If anybody is interested in will send the picture of
> those
> section.
>
>
>
>
>
> with regards,
>
> Aazathraj.P
>
> Technical Officer,
>
> Apollo Hospitals-chennai
>
> India.
>
> aazath <@t> hotmail.com
>
>
>
> _________________________________________________________________
> The latest in fashion and style in MSN Lifestyle
> http://lifestyle.in.msn.com/_______________________________________________
>
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
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> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
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> ------------------------------
>
> Message: 5
> Date: Mon, 26 Jul 2010 09:07:57 -0700 (PDT)
> From: kristen arvidson <arvidsonkristen <@t> yahoo.com>
> Subject: [Histonet] Special Stain Storage
> To: histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <820784.89949.qm <@t> web65707.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hello All,
> We were recently inspected by CLIA and our inspector noticed that we didn't have reagent storage temperatures written in our Special Stains procedures. We do our stains by hand so we do have some stored in the refrigerator and others stored at room temp. I went through some of the Histo books and I cannot find any specifics on storage of reagents. Any suggestions??
>
> Thanks!!
> Kristen
>
>
>
>
> ------------------------------
>
> Message: 6
> Date: Mon, 26 Jul 2010 12:53:38 -0400
> From: "Podawiltz, Thomas" <tpodawiltz <@t> lrgh.org>
> Subject: RE: [Histonet] Special Stain Storage
> To: 'kristen arvidson' <arvidsonkristen <@t> yahoo.com>, histonet
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <38667E7FB77ECD4E91BFAEB8D986386323D5DF832B <@t> LRGHEXVS1.practice.lrgh.org>
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> That is a new one on me. It has never come up during any of our inspections.
>
>
> Tom Podawiltz HT (ASCP)
> Histology Section Head/Laboratory Safety Officer
> LRGHealthcare
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of kristen arvidson
> Sent: Monday, July 26, 2010 12:08 PM
> To: histonet
> Subject: [Histonet] Special Stain Storage
>
> Hello All,
> We were recently inspected by CLIA and our inspector noticed that we didn't have reagent storage temperatures written in our Special Stains procedures. We do our stains by hand so we do have some stored in the refrigerator and others stored at room temp. I went through some of the Histo books and I cannot find any specifics on storage of reagents. Any suggestions??
>
> Thanks!!
> Kristen
>
>
>
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> ------------------------------
>
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> End of Histonet Digest, Vol 80, Issue 30
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