[Histonet] Biocare P63 with Dako Flex detection

[Cynthia Pyse]

Cathy
Have you tried adding an EnVision FLEX mouse linker to your protocol.
Sometimes that will work.

Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cpyse <@t> x-celllab.com



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Cathy.Crumpton <@t> tuality.org
Sent: Wednesday, July 21, 2010 3:47 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Biocare P63 with Dako Flex detection


   Hi  all, I have ran into a troubleshooting dilemma and was wonderi=g
   if  anyone  else could help.  Is there anyone out there that uses the
Biocare  Medical  P63 concentrated antibody with the Dako TRS and Flex
   detec=tion  kit?  I am curious what dilution you use and if you have
   to  do  an=ything  special  for  the  P63 (extra long retrieval, DAB
   enhancer, etc). = am having problems getting a strong signal for the
   basal cells in prosta=e.  I am running it at 1:50 with 40 minutes in
   pH  9  retrieval  (which  =seems  too  long to me but does help).  A
   dilution with the same protoco= at 1:100 is way too light.

   Thanks,
    <=DIV>
   Cathy Crumpton HT(ASCP), Histology Lead
   Tuality Community Hosp=tal
   Hillsboro, OR 97123
   (503)681-1292
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