[Histonet] vanGieson-Trichrome staining protocol

[Bernice Frederick]

And it looks so pretty (VVG/TRI)- especially on lung.


Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
ECOGPCO-RL 
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rena Fail
Sent: Monday, July 19, 2010 12:47 PM
To: Doughty, Adele; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] vanGieson-Trichrome staining protocol

Are you perhaps refering to the Verhoff's Van Gieson, if you are trying to
stain 
elastic then  a trichrome it can be done.  


The silver should be cloudy like a slightly dirty window , not milky 
generally 
if you add enough ammonia to the silver and sodium hydroxide mixture to
render 
it completely clear then you will probably have added too much ammonia. 

Rena Fail



----- Original Message ----
From: "Doughty, Adele" <adoughty <@t> lrri.org>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Mon, July 19, 2010 10:29:19 AM
Subject: [Histonet] vanGieson-Trichrome staining protocol



Is there anyone out there that has done these two stains together? If
so, I need assistance with a protocol. Also I am doing a reticulum stain
and I am having a time with the ammoniacal silver solution, I can get
the solution clear but when it comes to the cloudy do they mean milky
cloudy? Thank you Adele 

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