[Histonet] Embedding multiple GI pieces on end in a paraffin block

[R J VAZQUEZ]

Hello,

The way I used to embed GI specimens is to place them on end on a stack of specimen bags that have solidified, since they are already in place, then you can pick them up quickly. I did this with sectioned arterial artieries or vas deferens this worked like a dream.

 

Robyn Vazquez
 
> Date: Fri, 16 Jul 2010 11:00:01 -0500
> From: mpence <@t> grhs.net
> To: kgrobert <@t> rci.rutgers.edu; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Embedding multiple GI pieces on end in a paraffin block
> CC: 
> 
> I have techs that embed 10-15 pieces on end in one block. Just cool the
> block slowly and move your pieces around quickly.
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> kgrobert <@t> rci.rutgers.edu
> Sent: Friday, July 16, 2010 10:24 AM
> To: histonet
> Subject: [Histonet] Embedding multiple GI pieces on end in a paraffin
> block
> 
> 
> Is there a way to do this without one or more pieces falling over? I
> saw in the archive the method for frozen sections-embed them on their
> sides in OCT, then cut on the end, but I don't think I'd be able to do
> that in paraffin. Would one of the tissue microarray methods work?
> (I've never done that before, so I have no idea.)
> 
> Thanks in advance for all your help,
> Kathleen
> 
> 
> Principal Lab Technician
> Neurotoxicology Labs
> Molecular Pathology Facility Core
> Dept of Pharmacology & Toxicology
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