[Histonet] massons question

BSullivan <@t> shorememorial.org BSullivan <@t> shorememorial.org
Fri Jul 2 05:36:49 CDT 2010


Bouin's is used as a mordant in this stain.

Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
AP Supervisor
Shore Memorial Hospital
609-653-3590


                                                                           
             mohamed abd el                                                
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                                       [Histonet] massons question         
                                                                           
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hi all
i'm going to stain some slides of small intestin which were fixed in susa.
i'm going to stain it by Massons trichrome.
i have read that slides should be secondry fixed in Bouin's or saturated
picric solution. 
does it mean fixation of processed tissue after cutting with microtom??
how long??
could you please share me your protocol for massons 
thanks in advance 
mohamed
histology dep.
faculty of vet. med.
cairo univ.- Egypt



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