[Histonet] massons question

[BSullivan <@t> shorememorial.org]

Bouin's is used as a mordant in this stain.

Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
AP Supervisor
Shore Memorial Hospital
609-653-3590


                                                                           
             mohamed abd el                                                
             razik                                                         
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                                       [Histonet] massons question         
                                                                           
             07/02/2010 05:23                                              
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hi all
i'm going to stain some slides of small intestin which were fixed in susa.
i'm going to stain it by Massons trichrome.
i have read that slides should be secondry fixed in Bouin's or saturated
picric solution. 
does it mean fixation of processed tissue after cutting with microtom??
how long??
could you please share me your protocol for massons 
thanks in advance 
mohamed
histology dep.
faculty of vet. med.
cairo univ.- Egypt



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