[Histonet] massons question

BSullivan <@t> shorememorial.org BSullivan <@t> shorememorial.org
Fri Jul 2 05:36:49 CDT 2010

Bouin's is used as a mordant in this stain.

Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
AP Supervisor
Shore Memorial Hospital

             mohamed abd el                                                
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                                       [Histonet] massons question         
             07/02/2010 05:23                                              

hi all
i'm going to stain some slides of small intestin which were fixed in susa.
i'm going to stain it by Massons trichrome.
i have read that slides should be secondry fixed in Bouin's or saturated
picric solution. 
does it mean fixation of processed tissue after cutting with microtom??
how long??
could you please share me your protocol for massons 
thanks in advance 
histology dep.
faculty of vet. med.
cairo univ.- Egypt

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