[Histonet] massons question
llewllew <@t> shaw.ca
Fri Jul 2 10:46:33 CDT 2010
SuSa has a high mercuric chloride content, and tissues fixed in high mercury
fixatives do not require secondary fixation in picric acid (Bouin). The
mercuric chloride fixes in a manner which improves staining with acid dyes.
For Masson's trichrome on tissues fixed with simple formalin variants,
secondary fixation in Bouin or picric acid at 60C for an hour is recommended
after baking on and dewaxing.
----- Original Message -----
From: "mohamed abd el razik" <k84as <@t> yahoo.com>
To: <Histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, July 02, 2010 2:23 AM
Subject: [Histonet] massons question
i'm going to stain some slides of small intestin which were fixed in susa.
i'm going to stain it by Massons trichrome.
i have read that slides should be secondry fixed in Bouin's or saturated
does it mean fixation of processed tissue after cutting with microtom??
could you please share me your protocol for massons
thanks in advance
faculty of vet. med.
cairo univ.- Egypt
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