[Histonet] massons question

[Bryan Llewellyn]

SuSa has a high mercuric chloride content, and tissues fixed in high mercury 
fixatives do not require secondary fixation in picric acid (Bouin).  The 
mercuric chloride fixes in a manner which improves staining with acid dyes.

For Masson's trichrome on tissues fixed with simple formalin variants, 
secondary fixation in Bouin or picric acid at 60C for an hour is recommended 
after baking on and dewaxing.

Bryan Llewellyn

----- Original Message ----- 
From: "mohamed abd el razik" <k84as <@t> yahoo.com>
To: <Histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, July 02, 2010 2:23 AM
Subject: [Histonet] massons question


hi all
i'm going to stain some slides of small intestin which were fixed in susa.
i'm going to stain it by Massons trichrome.
i have read that slides should be secondry fixed in Bouin's or saturated 
picric solution.

does it mean fixation of processed tissue after cutting with microtom??
how long??
could you please share me your protocol for massons

thanks in advance

mohamed
histology dep.
faculty of vet. med.
cairo univ.- Egypt



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