[Histonet] massons question

mohamed abd el razik k84as <@t> yahoo.com
Fri Jul 2 04:23:27 CDT 2010

hi all
i'm going to stain some slides of small intestin which were fixed in susa. 
i'm going to stain it by Massons trichrome. 
i have read that slides should be secondry fixed in Bouin's or saturated picric solution.
does it mean fixation of processed tissue after cutting with microtom??
how long??
could you please share me your protocol for massons
thanks in advance
histology dep.
faculty of vet. med. 
cairo univ.- Egypt


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