[Histonet] massons question

[mohamed abd el razik]

hi all
i'm going to stain some slides of small intestin which were fixed in susa. 
i'm going to stain it by Massons trichrome. 
i have read that slides should be secondry fixed in Bouin's or saturated picric solution.
 
does it mean fixation of processed tissue after cutting with microtom??
how long??
could you please share me your protocol for massons
 
thanks in advance
 
mohamed
histology dep.
faculty of vet. med. 
cairo univ.- Egypt