[Histonet] Problems with MSB stain

[Stephen.Eyres <@t> sanofi-aventis.com]

Hi,
 
I have just encountered a problem I have never seen before and would
welcome opinions as to the cause. I work in pharma R&D and had a request
to perform MSBs on some liver slides. The results varied within the run,
with a distinct difference in the overall colour of the  slides; some
appearing blue and others red. The person performing the staining is
experienced, and our neqas scores are good. When we investigated the
problem the only difference we could identify was that the bluer slides
were stained after  40 minutes drying after being cut, whereas the
redder slides were cut the day before. This suggests that maybe slides
were incompletely dried and that this affected the staining. Comments
please.
 
Cheers
 
Steve