[Histonet] Counterstain for fluorescent tissue
John Kiernan
jkiernan <@t> uwo.ca
Sat Jan 9 00:46:20 CST 2010
Dear Nick Evans,
First: Say who and where you are, and who is in charge of your experiments with mice.
Second: Tell your boss to buy two or three histotechnology textbooks (about $50 each) and allow himself and you and all your colleagues 30 mins paid daily reading/lunch time.
John Kiernan
Anatomy, UWO
London, Canada
= = =
----- Original Message -----
From: Nicholas David Evans <ndevans <@t> stanford.edu>
Date: Friday, January 8, 2010 20:18
Subject: [Histonet] Counterstain for fluorescent tissue
To: histonet <@t> lists.utsouthwestern.edu
> Dear all,
>
>
>
> I am sorry if this is a bit of a basic question. I would like to
> observelocalization of fluorescent microparticles embedded in
> mouse skin. I plan
> to do this simply by cryosectioning skin, mounting the tissue without
> fixation, and observing under the fluorescence microscope (the
> particlesare added before killing the mouse).
>
>
>
> However, I would also like to counterstain the tissue without
> losing the
> fluorescence of my sample (so I can see similar features as I
> might using
> H&E). Can anyone suggest a good way to do this? I would be very
> gratefulfor any advice.
>
>
>
> Many thanks
>
> Nick Evans
>
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