[Histonet] Counterstain for fluorescent tissue

[John Kiernan]

Dear Nick Evans,
 
First:  Say who and where you are, and who is in charge of your experiments with mice. 
 
Second:  Tell your boss to buy two or three histotechnology textbooks (about $50 each) and allow himself and you and all your colleagues 30 mins paid daily reading/lunch time.
 
John Kiernan
Anatomy, UWO
London, Canada
= = =
----- Original Message -----
From: Nicholas David Evans <ndevans <@t> stanford.edu>
Date: Friday, January 8, 2010 20:18
Subject: [Histonet] Counterstain for fluorescent tissue
To: histonet <@t> lists.utsouthwestern.edu

> Dear all,
> 
>  
> 
> I am sorry if this is a bit of a basic question. I would like to 
> observelocalization of fluorescent microparticles embedded in 
> mouse skin. I plan
> to do this simply by cryosectioning skin, mounting the tissue without
> fixation, and observing under the fluorescence microscope (the 
> particlesare added before killing the mouse).
> 
>  
> 
> However, I would also like to counterstain the tissue without 
> losing the
> fluorescence of my sample (so I can see similar features as I 
> might using
> H&E). Can anyone suggest a good way to do this? I would be very 
> gratefulfor any advice.
> 
>  
> 
> Many thanks
> 
> Nick Evans
> 
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