[Histonet] Counterstain for fluorescent tissue

Nicholas David Evans ndevans <@t> stanford.edu
Fri Jan 8 19:17:31 CST 2010


Dear all,

 

I am sorry if this is a bit of a basic question. I would like to observe
localization of fluorescent microparticles embedded in mouse skin. I plan
to do this simply by cryosectioning skin, mounting the tissue without
fixation, and observing under the fluorescence microscope (the particles
are added before killing the mouse).

 

However, I would also like to counterstain the tissue without losing the
fluorescence of my sample (so I can see similar features as I might using
H&E). Can anyone suggest a good way to do this? I would be very grateful
for any advice.

 

Many thanks

Nick Evans



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