[Histonet] Counterstain for fluorescent tissue

[Nicholas David Evans]

Dear all,

 

I am sorry if this is a bit of a basic question. I would like to observe
localization of fluorescent microparticles embedded in mouse skin. I plan
to do this simply by cryosectioning skin, mounting the tissue without
fixation, and observing under the fluorescence microscope (the particles
are added before killing the mouse).

 

However, I would also like to counterstain the tissue without losing the
fluorescence of my sample (so I can see similar features as I might using
H&E). Can anyone suggest a good way to do this? I would be very grateful
for any advice.

 

Many thanks

Nick Evans